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Bacterial Growth and Decay - Occidental College Occidental 细菌的生长和衰变-西方学院西方.pptVIP

Bacterial Growth and Decay - Occidental College Occidental 细菌的生长和衰变-西方学院西方.ppt

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Bacterial Growth and Decay - Occidental College Occidental 细菌的生长和衰变-西方学院西方.ppt

Facts about Escherichia Coli (E. Coli) E. Coli grows well between 21 degrees Celsius to 49 degrees Celsius with an optimum at about 37 degrees Celsius The growth and decay rate are also affected by: Temperature Initial concentration of Bacteria Presence of antibacterial substances pH levels Oxidation reduction potential In our experiment, we demonstrate how K value is affected by temperature Objectives / Thesis Examine and construct a model that represents bacteria’s behavior Compare K values from natural decay to K values due to Temperature change Chemical Poisoning Compare logistic vs. exponential decay from above the carrying capacity Our Model: Description of Bacteria behavior Bacteria gradually grow to a certain point (lag phase) They start to grow exponentially (exponential growth phase) The population then approaches the carrying capacity (stationary phase) Die off at a particular rate (death or logistic decline phase) Model Conditions B(0) = 1000 bacteria in Petri dish with glucose at 37 degree Celsius Carrying capacity= 100,000 K- value found from research specific to 37 degrees Celcius conditions Logistic Growth Used to model the first three phase of the graph Equation: Where K is 0.00029 Results of Logistic Growth Population approaches carrying capacity at t= 55, 544.95 seconds = 15 hours Exponential Decay Equation: B(t)=99999e^-0.00029t Results of Exponential Decay Takes 71,459.5 seconds (19.8 hours) to reach a population of 0.0001 Rounded to the nearest bacterium population = 0 This is 4.58 hours longer than the bacteria took to reach the carrying capacity in Logistic growth How does changing the K value affect Bacteria Population? We solved for time that would take the population to decay from 99999 to 0.0001 using K values both greater and smaller than our previous value of 0.00029. Greater K value decays quicker Ratio of K value to our initial K value proportionate to the decrease to the time necessary for the population to decay

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