父源性印记基因在牛早期胚胎中的表达-中国农业科学.pdfVIP

中国农业科学 2013,46(18):3887-3893 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.18.019 父源性印记基因在牛早期胚胎中的表达 张春强, 赵德超, 韩志玲, 张文广, 张家新 （内蒙古农业大学动物科学学院/动物遗传育种与繁殖自治区重点实验室，呼和浩特 010018 ） 摘要：【目的】研究父源印记基因在牛早期胚胎中的表达模式。【方法】利用Real-time PCR 技术，检测Gnas、 Grb10 和Xist 这3 个父源性印记基因在牛体外受精（In vitro Fertilization，IVF）和孤雌激活（Parthenogenetic Activation，PA）胚胎各发育阶段的表达情况。【结果】对于IVF 和PA 两种来源的牛胚胎，Gnas 在胚胎各发育阶 段的表达没有明显差异；Grb10 在2 细胞阶段的PA 胚胎中的表达量明显高于在IVF 胚胎的，但从4 细胞阶段到囊 胚阶段，Grb10 在两种来源的牛胚胎中表达没有明显差异；从2 细胞阶段到8 细胞阶段，Xist 在两种来源的牛胚 胎中表达没有明显差异，但从 16 细胞期开始，Xist 在PA 胚胎中的表达明显高于 IVF 胚胎的表达量。【结论】对 于牛IVF 胚胎和PA 胚胎，Gnas、Grb10 和Xist 从2 细胞期到囊胚期都可以检测到表达，但表达模式不同。这3 个父源性印记基因在牛胚胎发育过程中可能发挥着重要的作用。 关键词：牛；父源性印记基因；早期胚胎；表达 The Expression of Paternal Imprinting Genes in Bovine Early Stage Embryo ZHANG Chun-qiang, ZHAO De-chao, HAN Zhi-ling, ZHANG Wen-guang, ZHANG Jia-xin (1 The Key Lab of Animal Genetics, Breeding, and Reproduction/ The College of Animal Science of Inner Mongolia Agricultural University, Hohhot 010018) Abstract: 【Objective 】The objective of the experiment is to investigate the expression pattern of paternal imprinting genes in bovine early stage embryo. 【Method 】The expression profile of paternal imprinting genes Gnas, Grb10, and Xist were detected in bovine embryo derived from in vitro fertilization (IVF) and parthenogenetic activation (PA) by real-time PCR. 【Result 】There were no difference in imprinted expression profile of Gnas between IVF and PA embryo. Grb10 expression was significantly higher during 2-cell stage in PA embryo than that of IVF embryo, however, no difference was observed in expression of Grb10 between IVF and PA embryos from 4-cell satge to blastcyst stage. Xist expression was similar