2006年2010年山东省鸡传染性支气管炎病毒分离株基因型和血清型研究 word格式.docxVIP

优秀毕业论文 精品参考文献资料 2006—2010 年山东省鸡传染性支气管炎病毒分离株基因型和血清型分析 genetically relatively parallel and closely related to strain LX4. However, Genetic recombination may have happened in three isolates SDTA06111, SDWF0608 and CK/CH/SD09/005, as their S1, N and M genes belong to different evolutionary branch. The resuLts showed that point mutation, insertion and recombination may have contributed to the emergence of the variant IBV strains at the immune pressure. The neutralization tests result showed that SDWF0608, SDLY0612, SDLY0701 with the vaccine strains H120 belong to the same subtypes of Mass serotypes, the other five isolates with H120 and 491 do not belong to the same serotype, the antigen relatedness values less than 25% (belonging to different serotypes). The results from the perspective of humoral immunity in chickens immune to explain the clinical H120 or 491 IBV vaccine is still frequent cause of the infection, but also prompted IBV strains present muLtiple serotypes in Shandong Province. Based on the type of serum of these epidemic strains, multivalent vaccine must be produced. S1 gene sequence with antigenic relatedness results show that the H120 and strain SDTA06111were clustered into gene I, and the S1 gene nucleotide sequence homologies were 99.3%, but the R between the two strains only 18% ; with the H120 strain of SDLY0612 highestantigen relatedness value (70.71%), but both S1 gene sequence homology of only 78.2%, indicating that combined with S1 gene sequence analysis, their resuLts can not be completely correlation, IBV can lead to neutralizing epitope changes by a small number of mutations , resuLting in a new serotype. In summary, the IBV gene mutation, insertion, deletion and recombination continue to generate new serotypes, resuLting in the production of vaccine failure, we must strengthen the monitoring of epidemic strains of IBV and take including biosafety, including the comprehensive prevention and control measures can effectively prevent