2型糖尿病模型猪肾脏tlr4表达与血清tgfβ1 mcp1含量变化的研究 word格式.docxVIP

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2型糖尿病模型猪肾脏tlr4表达与血清tgfβ1 mcp1含量变化的研究 word格式.docx

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2型糖尿病模型猪肾脏tlr4表达与血清tgfβ1 mcp1含量变化的研究 word格式

目录中文摘要·······························································································（1）英文摘要·······························································································（3）论文题目·······························································································（5）材料与方法····························································································（8）结果···································································································（12）讨论···································································································（18）结论···································································································（22）参考文献·····························································································（23）文献综述·····························································································（27）参考文献·····························································································（37）缩略语表·····························································································（45）攻读学位期间发表文章及科研情况····························································（46）个人简历·····························································································（47）致谢···································································································（48）中文摘要目的：观察高糖高脂饲养建立的2型糖尿病模型猪肾脏病理TLR4(TollLikeReceptor 4，TLR4)的表达，并探讨其与造模过程中血清单核细胞趋化蛋白-1（Monocyte ChemoattractantProtein-1，MCP-1）、转化生长因子β1（TransformingGrowth Factor-β1，TGF-β1）含量变化及血糖的关系，探讨上述因子在早期糖尿病肾病中的作用及可能机制。材料和方法：将8头2型糖尿病（Type2DiabetesMellitus，T2DM）模型猪依据病理诊断分为2组；糖尿病组（DiabetesMellitus组，DM组）5头、糖尿病肾病组（Diabetic Nephropathy组，DN组）3头，对照组（Control Diet组，CD 组）10头，一共3组。采用ELISA法测定0、3、6月末TGF-β1、MCP-1血清浓度，免疫荧光法观察6月末肾脏TLR4表达。对比3组间TGF-β1、MCP-1血清浓度的变化，以及TLR4在肾组织中表达变化。三组数据间比较采用单因素方差分析，TGF-β1、MCP-1、血糖之间相关性，采用spearman相关分析法。结果：1、DM组及DN组MCP-1血清浓度呈逐渐增加趋势（DM（ng/L）/DN（ng/L）:48.93±3.52→71.76±2.77→95.37±3.10