不同品种菊花成分分析及挥发油分析-药物分析学专业论文 word格式.docxVIP

were no sucrose and maltose. The total contents of free sugars in Flos chrysanthemi were in the range of 9.01 ～18.76 g/100g. The highest content of total free sugars was found inGongju, while the lowest content in Huangju The total levels of free amino acids in Flos chrysanthemi range from 2.806 to 0.563 g/100g, among which Qi yueju showed the highest level of total free amino acids, Hang baiju showed the lowest level of total free amino acids.The fingerprint analysis method was established to research on the volatile constituents of Gong Chrysanthemum morifolium in Huangshan City by using GC-MS and Computer Aided Similarity Evaluation. The results showed that the optimum condition of GC-MS was applied with a fiber column (0.25mm*30m*0.25μm) coated withHP-5MS.The temperature at feed inlet was 260℃, the carrier gas was nitrogen, the flowrate was 1.0mL·min-1, the split ratio was 25:1, and the sample size was 2μL. The essential oil in ten batches of Gongju was analyzed by adopting temperature programmer. In this condition, 59 common peaks were identified and GC-MS Fingerprint of 59 common peaks was established preliminarily. The correlation coefficients of ten batches of Gongju were higher than 0.9. The content of Bicyclo[3.1.1]hept-2-en-4-ol-2,6,6-trimethyl-,aceate in volatile oil of Gongju was highest (20%). The method is of high degree of precision, reproducibility, and stability. The separating degree of each component in the essential oil is good. Both the established fingerprints and the component of Bicyclo[3.1.1]hept-2-en-4-ol-2,6,6-trimethyl-,aceate could be used as the quality control indexes for Gong Chrysanthemum morifolium in Huangshan City.From Jin zhuling Gongju, Shangfeng Gongju, Shi menhe Gongju, Jin gongju , Qi yueju and Jin xiangju 199、206、201、199、223 and 237 chromatographic peaks were detected, among them 49、94、106、96、121 and 110 components were iden tified, which were composed about 89%、66%、82%、75%、82% and 77%