不同途径移植mscs联合小间隙吻合法修复周围神经损伤的实验研究-外科学(骨外科学)专业论文 word格式.docxVIP

不同途径移植MSCs联合小间隙吻合法修复周围神经损伤的实验研究中文摘要性染色的干细胞，且局部雪旺细胞增生活跃；○6电镜下的超微结构，CG组髓鞘形状不规则，板层厚度较薄，部分可见脱髓鞘改变及轴突变性，雪旺细胞增生不明显。VG组髓鞘形状不规则，板层厚度较薄，未见神经纤维脱髓鞘性改变，雪旺细胞增生明显。GG组髓鞘呈圆形或椭圆形，形态规则，板层排立致密、较厚，无脱髓鞘改变及轴突变性。结论：1、通过静脉和肌肉两种途径移植的骨髓间充质干细胞能归巢至周围神经损伤部位；2、通过尾静脉和腓肠肌两种途径移植骨髓间充质干细胞均能促进神经的再生和功能的恢复，且腓肠肌途径优于尾静脉途径；3、通过腓肠肌途径移植骨髓间充质干细胞能够延缓失神经支配肌肉的萎缩。关键词：骨髓间充质干细胞；小间隙；移植；神经再生；归巢作者：钱源源指导老师：王培吉英文摘要不同途径移植MSCs联合小间隙吻合法修复周围神经损伤的实验研究Experimental studyon peripheralnerveinjurybydifferent ways transplantationof MSCswithsmallgapanastomosis neurorrhaphy AbstractPart1Theseparation,cultureandidentificationofBonemarrow mesenchymalstemcellsObjective：Tomasterthemethodfortheisolation,cultureandamplificationofratbone marrow mesenchymal stem cells(BMSCs) in vitro and explore their biological characteristics.Methods：BMSCswerecollectedfromdegermedfemurs,andtibiasofSDratbyflushingtheshaft withL-DMEMusingasyringe.Cellsweredisaggregatedbygentle pipettingseveraltimesandplatedincultureflaskandre-fedevery2~3days(L-DMEMwith 10％FBS).When 90％fusion, cells were digested with trypsogen2 ~3 min andpassaged. Aftersuccessiveisolation,purification,subcultureandproliferation,the morphologywasobservedwithphasecontrastmicroscope.ThegrowthcurveofP2,P4,P6 weredrawnbycellcounting.TheproliferationandmorphologyofBMSCswasobserved withinvertedmicroscopeconstantly.Cells wereidentifiedbyFlowCytometry.Results：ThecomponentsofprimarilyculturedBMSCswereverycomplex.Themarrowcells were roundinthebeginningandafewcells adheredtoflaskwithinfirst24h，whichwereinirregularshapesuchasfusiform,polygonandsoon.Thefirst2to5days the proliferationofcellswereslowly.About6~9dayslater,cellsmightovergrowthebottom ofcultureflask.Thecellsarrangedregularlyasawhirlpool.Cellscouldspreadthefull flaskbottomfor12or15days.Generatedcellsstuckonthewallmorequicklythan primarycells.Thecellmorphologywasmoreuniformandallthecellsarrangedmore regularlyaftergeneratedby4or5times.ThegrowthcurveofP2,P4,P6werequitesimilar andthecellsbiologicalcharacterkeptstable.Theresultofgrowthcurveshowedtha