药物代谢动力学研究_外文文献原稿和译文毕业论文（可编辑）.docVIP

药物代谢动力学研究_外文文献原稿和译文毕业论文 Pharmacokinetic study of six flavones in rat plasma and tissues after oral administration of ‘JiangYaBiFeng’ using SPE -HPLC -DAD Abstract In this study, a high performance liquid chromatography HPLC coupled with diode array detection DAD for simultaneous determination of six flavones including baicalein, sophoricoside, rutin, baicalin, quercetin and genistein in rat plasma and tissues after oral administration of JiangYaBiFeng JYBF tablets was developed. The investigated analytes in plasma and tissues were extracted and purified with liquid -liquid extraction and solid phase extraction SPE. Chromatographic separation was accomplished on a DIONEX Acclaim C18 column 250mm×4.6mm, 5.0μm particle size with a simple linear gradient elution. The calibration curves for all the flavones had good linearity in the measured range with R2 higher than 0.9983. The relative errors REs of the intra? and inter?day accuracy at different flavones levels were all less than ±10%. The proposed method enables unambiguous identification and quantification of investigated flavones in vivo. This is the first report on determination of the major flavones in rat plasma and tissues after oral administration of JYBF tablets. The result provided a meaningful basis for evaluating the clinical application of this medicineKeywords: JiangYaBiFeng tablet; Flavones; HPLC?DAD; Rat plasma; Tissue distribution 1. Introduction ‘JiangYaBiFeng’ tablet JYBF tablet, a new drug for the treatment of hypertension has been widely used in the clinic in china because of its good effect and small side effects. It is composed of two western medicines pargyline and hydrochlorothiazide and three traditional Chinese medicines TCMs, including Scutellaria baicalensis, Sophora japonica and Arachis hypogaea. According to some reported papers, flavones, such as baicalin, baicalein, sophoricoside, rutin, quercetin and genistein originally from the three TCMs are the main effective components conta