几丁糖聚乙烯醇神经导管修复周围神经缺损的实验研究-外科学专业论文 word格式.docx

AbstractPart oneEvaluation of chitosan/ poly (vinyl alcohol) nerve guidance conduits used for peripheral nerve regeneration: an experimental study in ratsPurpose: In the present study, poly(vinyl alcohol) (PVA) was added to chitosan to form acomposite material, with improved mechanical properties, for use as nerve conduits by the method of repeated freezing-thawing.We examined the effects of chitosan/polyvinyl alcohol nerve conduit in repairing peripheral nerve defect at different time.Methods: Eighty-four adult male Sprague–Dawley (SD) rats weighing 180-220 g were randomly divided into four nerve grafting groups, with eleven rats in each group. Chitosan/poly (vinyl alcohol) nerve guide conduit group, poly(lactic-co-carprolactone) tubes, silicon tubes and autografts group respectively, (groups A, B and C; control group D). A segment of left sciatic nerve was resected and removed, leaving a 15-mm long defect following retraction of the nerve ends at all rats. The defects were bridged by chitosan/poly (vinyl alcohol) nerve guide conduit, poly(lactic-co-carprolactone) tubes, silicon tubes andautografts group respectively. Four-week, 8-week and 12-week postoperative，a series ofexaminations was performed, including Sciatic Functional Index (SFI), electrophysiological methods, Weight of the gastrocnemius and soleus muscles, and histological examination. 12-week postoperative, True Blue retrograde tracing was examined.Results: In experimental and control groups, all rats showed no noticeable signs of systemic or regional inflammation and serious surgical complications after implantation and the locomotor function of their injured limbs gradually recovered. Three rats developed mild decubital in their left heel in the chitosan/PVA group, 4 rats in the P(LLA-CL) group, 6 in the silicon group, and 2 in autograft group because of the sciatic nerve injury at two weeks post-surgery. At 4 weeks postoperative, the chitosan/PVA tube showed degradation. The regenerated nerve have br