凝胶图像间的蛋白点匹配方法研究-通信与信息系统专业论文 word格式.docxVIP

AbstractWiththecompletionofthedeterminationofhumangenomesequence,anew era-post-genomiceraiscoming.Thekeytaskoflifesciencehasbeentransferredfrom sequencingofgenometothefunctionalcharacterizationofgenes.Proteomicsisthe importantcomponentofthepost-genomic era,andthetwo-dimensionalgelimage analysistechniqueisoneoftheimportanttechniquesinproteomics.Extractionand analysisofvariantproteinspotsisakeycontentinthetwo-dimensionalgelimage analysistechnique,andproteinspotmatchingisthekeylinkinvariantproteinspots extracting.Proteinspotmatchingcanbeusedtoidentifypotentialvariantprotein molecules,andprovideevidencefordiseasediagnosis,drugresearchandcontamination analysis.Bytakingproteinspotofthegelelectrophoresisimageastheresearchobject,we explorethealgorithmofproteinspotmatchingbasedonthegel’simagesinthispaper. Themain research work,results andinnovation points are as follows:Firstly,itintroducedtheproteomicsanditskeytechnology,explainedthe backgroundandsignificanceofresearch,reviewedthealgorithmofproteinspot matchingbased on thegel images.Secondly,throughtheuseoftheadditionallandmarksandthenear-neighborhood criteria,itpresentedasemi-automaticmatchingalgorithmbasedonlandmarksingel images.Regiondivisionandspotmatchingincorrespondingregionandcross-region haveeffectivelyraisedthealgorithm’s accuracy,andrespectivelyconfirm the effectivenessofthealgorithmfromlandmarknumberandimagesource.Butthis method was semi-automatic, needed hand work.Thirdly,basedonthecharacteringoffeaturespotsdistributioningelimages,it presentedanautomaticmatchingalgorithmwitchfirstfeaturespotsmatchthenspots accuracymatchingelimages.Atthebeginning,calculatedthematchthresholddistance withstatisticstheory,thenfeaturespotswereextractedandmatchedbasedonregion divisionandsimilarity.afterthat,calculatedthetransformationparameterbetweentwo imageswiththematchedfeaturespots.lastly,spotswerematchedaccuratelyingel images.Theeffectiveness of the algorithm was confirmedwith realgel images.Finally,forbettermedicaleffect,itprese