利用基因芯片分析拉米夫定治疗过程中hbvdna的基因变异.pdfVIP

维普资讯 第 l8卷6期 中 国 病 毒 学 18 (6)：523- 529 2003年 12月 VIR0L0GICA SINICA December 2003 利用基因芯片分析拉米夫定治疗过程 中HBVDNA的基因变异 高英堂1,2 陈瑞阳1，宋文芹 ，陈成彬 1，齐之丽2 景 丽2孙金英2钱绍诚2 (1．南开大学生命科学学院，天津 ，300071：2．天津市第三中心医院，天津，300170) DNA M icroarrayforM onitoringGeneticVariabilityofHepatitisB virus duringLamivudineTherapy GAOYnig—tang，CHENRui—yang ，SONGWen—qin 。 CHENCheng．bin，QIZhi．1i， JINGLi，SUNJin．ying。QIANShao—cheng rJ．Collegeoflfiesciences,NankaiUniversity,Tianjin300071，China；2．TianjinThirdCentralHospital,Tmnjin300170，China) Abstract：TheHepatitisB virus(HBV)oligochipwasmadeaccordingtothesequenceofHBV polymerasegene．7genotypesand4sero—subtypesofHBV,aswellaspositionrtV173，~．L180，rtlVl21M， rtV207inhtereversetranscriptase(rt)domainofHBV polymerase，weredetectedwiht htechip．45 patientsweredivided intoA nadB groupsaccordingtohteirALT levels．Serum samplesforchip naalysiswereobtainedat0，3，6，9，12monhtsoftreatment．Among45patients，39weregenotype Cnad subtype adr,6were genotypeB nadsubtypeadw．Among38patientswhom were rteatde continuously ofr12monhts，1lamivudineresistantmutnatwasdiscoveredin 17ofA groupwiht highATL level，4 varinatswere momtored in21ofB groupwiht normalALT leve1．Allvarinats werertM 204V nad rtL180M ，2ofhtem were mixedwiht HBV wildtype ．Th ertM 204V mutnatwasfoundat6monhtsof htempy,hte~L180M mutantwasdetectedafterward．Th eresultsobtainedbysequencingofhte10PCR productsnadchiparrayingwere almosthtesame，hteonlydifferentwashtat1varinatatpositionrtV173 Was notdetected by htegenechip．Furthernaalysing HBV DNA values，ALT levelsnadHBeAg seroconversioninrelationtoHBV mutants，hteresultsshowedhtatamore rapidoccurrenceofvarina t Was