乌拉尔图小麦WRKY转录因子的筛选与分析-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2015, 41(6): 900909 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2015.00900 乌拉尔图小麦WRKY 转录因子的筛选与分析 马建辉 张黛静 高小龙 邵 云 姜丽娜* , 453007 : WRKY , A WRKY , WRKY , 62 WRKY , 14 , 2 WRKY ; , 62 WRKY 8 , , 2 WRKY , : ; WRKY ; ; Identification and Analysis of WRKY Transcription Factors in Triticum urartu * MA Jian-Hui, ZHANG Dai-Jing, GAO Xiao-Long, SHAO Yun, and JIANG Li-Na College of Life Sciences, Henan Normal University, Xinxiang 453007, China Abstract: WRKY transcription factors have been found to be involved in the processes responding to various abiotic and biotic stresses in plants. The knowledge on WRKY transcription factors in Triticum urartu (AA) will facilitate the function study of WRKY transcription factors in hexaploid wheat (Triticum aestivum, AABBDD). In this study, 62 WRKY transcription factors with full length of coding sequence (CDS) were selected from Triticum urartu genome through bioinformatic analyses, in which 14 could be located on specific chromosomes and two pairs had been duplicated. These WRKY transcription factors were divided into eight subgroups by phylogenetic analysis and the exon–intron structure in individual subgroups was relatively conserved. Two WRKY transcription factors were selected for function validation, and their expressions consistently increased under diverse abiotic stresses by qRT-PCR assay. Keywords: Triticum urart