卵巢切除大鼠不同脑区酪氨酸激酶A表达的变化.docVIP

卵巢切除大鼠不同脑区酪氨酸激酶A表达的变化 【关键词】 卵巢 ［摘要］ 目的 观察卵巢切除大鼠脑内不同部位酪氨酸激酶A（TrkA）表达的变化。方法 按Singh方法建立大鼠卵巢切除模型（OVX组），免疫细胞化学染色，观察海马CA1区、皮质区、杏仁复合体区、基底前脑Meynert核等部位TrkA阳性细胞表达的变化。结果 对照组大鼠各观察区有大量TrkA表达，与对照组比较，OVX组大脑海马CA1区、大脑皮质区、杏仁复合体区TrkA阳性细胞数明显减少（t=5.90～8.57,P0.01），Meynert核区无显著性差异（t=1.65，P0.05）。结论 大鼠体内雌激素下降可致脑内多部位TrkA表达减少，雌激素与生长因子受体系统可能存在相互影响的关系。 ［关键词］ 酪氨酸激酶A；雌激素；大鼠，Wistar EXPRESSIVE VARIETY OF TYROSINE KINASE A IN DIFFERENT CEREBRAL REGIONS OF OVARIECTOMIZED RATSYANG XUEWEI，GUO ZONGJUN，LAN KETAO, et al (Institute of Cerebrovascular Diseases, Qingdao University Medical College, Qingdao 266003, China) ［ABSTRACT］ObjectiveTo observe the changes of tyrosine kinase A (TrkA) in different cerebral regions of ovariectomized rats. MethodsAdult healthy female Wistar rats were randomly divided into two groups: model group (OVX group) and control group. The positive expressions of TrkA cells in the hippocammpi CA1, cortex, amygdala, Meynert of the basal forebrain were compared between the two groups.ResultsThe positive TrkA cells were observed in every section of the brain in control group, while in model group，the positive TrkA cells in the hippocammpi CA1, cortex and amygdala were distinctly decreased as compared with the control group （t=5.90-8.57,P0.01）. No difference was found in the Meynert nucleus between the two groups. Conclusion The low estrogen induces decrease of positive expression of TrkA in various parts in ovariectomized rats brain. A correlation probably exist between estrogen and growthfactorreceptor system. ［KEY WORDS］tyrosine kinase A; estrogen; rats, Wistar 研究结果表明，雌激素受体（ER）系统与神经生长因子（NGF）受体酪氨酸激酶A(TrkA)在同一神经细胞上具有共存现象，二者之间可能存在共同的作用位点［1］。女性体内的雌激素缺乏与轻度认知障碍（MCI）和阿尔茨海默病（AD）密切相关［2］。本实验观察切除双侧卵巢(OVX)大鼠脑内皮质区、海马CA1区、杏仁复合体区、基底前脑Meynert核等部位TrkA阳性细胞表达的变化，旨在探讨雌激素与TrkA的关系。 1 材料和方法 1.1 实验动物与分组 成年健康雌性Wistar大鼠14只，由山东大学实验动物中心提供，5月龄，体质量210～250 g。常规饲养，环境温度22 ℃，随机分为OVX组和对照组，每组大鼠7只。 1.2 模型制作 按SINGH等［3］的方法制备大鼠OVX模型。对照组大鼠除了不切除卵巢以外,其余处理方法同OVX组。 1.3 大鼠脑组织病理观察 术后观察大鼠1个月后以100 g/L水合氯醛麻醉大鼠(300 mg/kg