大肠杆菌分类.docVIP

大肠杆菌分类 A listed gene name means that gene carries a loss of function mutation, a Δ preceding a gene name means the gene is deleted. If a gene is not listed, it is not known to be mutated. Prophages present in wt K-12 strains (F, λ, e14, rac) are listed only if absent. E. coli B strains are naturally lon- and dcm-. F-?= Does not carry the F plasmid F+?= Carries the F plasmid. The cell is able to mate with F-?through conjugation. F[ ]?= Carries an F plasmid that has host chromosomal genes on it from a previous recombination event. This cell can also mate with F-?through conjugation. Chromosomal genes carried in the F plasmid are listed in brackets. rB/K+/-?= The (B/K) defines the strain lineage. The +/- indicates whether the strain has or hasnt got the restriction system. mB/K+/-?= The (B/K) defines the strain lineage. The +/- indicates whether the strain has or hasnt got the modification (methylation) system. hsdS?= Both restriction and methylation of certain sequences is deleted from the strain. If you transform DNA from such a strain into a wild type strain, it will be degraded. hsdR?= For efficient transformation of cloned unmethylated DNA from PCR amplifications INV( )?= chromosomal inversion between locations indicated ahpC?= mutation to alkyl hydroperoxide reductase conferring disulfide reductase activity ara-14?= cannot metabolize arabinose araD?= mutation in L-ribulose-phosphate 4-epimerase blocks arabinose metabolism cycA?= mutation in alanine transporter; cannot use alanine as a carbon source dapD?= mutation in succinyl diaminopimelate aminotransferase leads to succinate or (lysine + methionine) requirement Δ( )?= chromosomal deletion of genes between the listed genes (may include unlisted genes!) dam?= adenine methylation at GATC sequences exist; high recombination efficiency; DNA repair turned on dcm?= cytosine methylation at second C of CCWGG sites exist. dam dcm are the default properties and always elided, while dam-?or dcm-?should be declare explicit