智能复合纳米颗粒用于诊断治疗一体化的应用研究-流行病与卫生统计学专业论文.docx

关键词：透明质酸纳米颗粒石墨烯金纳米颗粒光热治疗荧光成像光声成像万方数据AbstractIt iS well known that cancer has become a major problem m the field of human health．The clinical study find that early diagnosis and treatment are useful methods to control and treat tumor．It has become a hot topic in these research to achieve early diagnosis of tumors as well as improve the therapeutic effect．Nanomedicine，the application of nanotechnology in medicine，promotes development of liposomes， nanoparticles，micelles and other drug-loaded nanoparticles．Above all，these materials in nanomedicine as effective theranostic agents can be used for diagnosis and therapy of various diseases．An ideal nanomaterial will provide：(1)early visualization of tumors or other disease sites，(2)effective delivery of drugs into the site of interest，(3) distribution of the theranostic agent in vivo，and(4)optimized therapeutic strategy to reduce adverse side effects．Therefore，excellent nanomaterials play an important role in diagnosis and treatment fields．In the second chapter,we developed a multifunctional nanocomposite by loading copper sulfide(CuS)into Cy5．5·conjugated hyaluronic acid nanoparticles(HANP)， forming an activatable Cy5．5一HANP／CuS(HANPC)nanocomposite．CuS with strong NIR absorbance became an excellent contrast agent for photoacoustic(PAl)imaging and photothermal therapy(PTT)．In this system，Cy5．5 fluorescent signal was quenched by CuS inside the particle．when the whole nanocomposite was degraded by hyaluronidase，strong fluorescence signals were monitored in the tumor site．Afterintravenous administration of HANPC into SCC7 tumor-bearing mice，high fluorescence and PA signals were observed in the tumor site．In addition，tumors were then irradiated with a laser,and then an outstanding tumor inhibition rate was observed． In order to further improve the light conversion efficiency and tumor ablation effect ofnanomaterials．In the third chapter,we developed a fluorescent／PAI guided PTT agent by seeding Gold(Au)nanoparticles onto g