英文mulv实验方案.pdfVIP

Nov. 1, 2004. We need the laboratory of Basement, E 13, for the research on liver and kidney pathology in SAMP8 mice and other specific histostaining and immunostaining processes. The on going scientific research projects including: 1) The study of neurodegeneration in NFkB knockout mice, iNOS knockout mice and PrP knockout mice using Fluoro-Jade staining and specific silver staining techniques. 2) Time course of fatty deposition in SAMP8 mice using Oil red staining and Sudan black staining in vibratome section and/or cryostat section. 3) MulV virus immunostaining in vibratome section and/or cryostat section in the liver of SAMP8 mice. 4) MulV virus immunostaining in vibratome section and/or cryostat section in the kidney of SAMP8 mice. 5) Onset and dynamics of liver degeneration in SAMP8 mice induced by murine leukemia virus (MuLV). 6) Onset and dynamics of kidney degeneration in SAMP8 mice induced by murine leukemia virus (MuLV). We need the Cryostat for cutting sections at 15 um thickness and mounted directly on gelatin-coated slides. We need the vibratome for cutting sections at 50 um thickness and collected in buffer solution. We need the Tissue processor, the Microwave, Immunostaing tools, and other Histological staining tools in the room for our research projects. Fluoro-Jade is an anionic fluorochrome capable of selectively staining degenerating neurons in brain slices. The histochemical application of Fluoro-Jade results in a simple, sensitive and reliable method for staining degenerating neurons and their processes. Fluoro-Jade can detect neuronal degeneration results from a variety of neurotoxin, genetic defect, and virus infection. The block is freeze-sectioned (30-40 um) with a sliding microtome, resulting in free- floting sections. Xuemin Ye, Ph.D. Department of Virology Ex 5155 Histological stain Staining