细菌群体感应系统对合成聚羟基脂肪酸酯调控作用的研究-微生物学专业论文.docxVIP

优秀毕业论文 精品参考文献资料 合以上实验结果我们得出结论:在铜绿假单胞菌中，细菌群体感应系统促进胞内 PHA 积累，正向调控 P队的生物合成。 本实验首次提供了制绿假单胞商中群体感应系统对其生物合成 PHA 调指作用的直 接实验证据，丰富了群体感应系统的生物学功能，为设计适当的技术手段提高 PHA 的 产量和产率提供可能的理论指导，为提高 PHA 的生产提供了一定的理论依据。 关键词 z 群体感应聚经基脂肪酸酶钢绿假单胞菌 JV Abstract Many bacteria1 species control and modulate their physiology in response to increases in their population densities in a process known ωquorum sensing. QS，an important system， regulates different biological functions in bacteria. Polyhydroxyalkanoates (PHA) ，a family of intracellular polyesters synthesized by many bacteria under a specific physical conditions (such as excessive carbon， oxygen，phosphorus ， nitrogen，magnesium deficienc弘 etc.). Apart from the physicochemic a1 and mechanical properties comparable 由at of chemical polymeric materials，PHAs a1so have biodegradable ， biocompatible ，optica1 activity. PHAs have received increasing attentions because it can replace petrochemic a1 plastics. Does QS control the PHA synthesis in bacterial cells? In recent years，many experiment a1 results strongly hint that bacteria1 QS system is likely to be involved in the re肌llation of PHA，but no direct experimenta1 evidence is available so far. h也e present study，with the wild type Pseudomonas aeruginosa ωoriginal materia1， using physic a1 and. chemica1 means. and molecular biologica1阳chniques，出.e differences of PHA accumulation in wild type strain and its mutants defected with the syn也拙e gene for the individua1 signa1 molecule were analysed.lt has been found that:1) Compare with the PHA accumulation of wild type，the PHA content was reduced 15% in C4-HSL s归thase gene mutant PA0210 (e.rh的， 3，O. XO-C12-HSL s归由制e gene mutant PA0214 (61asl) and PA0216 (61as1/6rhll)reduced about 50%.ηlese indicated 由副 the quorum sensing system involved in the regulation of PHA biosynthesis and 也e lasIllαsR system played a m司or role; 2)ηle.PHA accumulation ofwild type PAOl ，PA0210 (6rh/l)and P