丝素蛋白材料的抗凝血性及其改性分析-纺织材料与纺织品设计专业论文.docxVIP

丝素蛋白材料的抗凝血性及其改性研究英文摘要Anticoagulation and modification of silk fibroin material AbstractCardiovascular disease has become a big threat to human health, the /search?q=problemamp;keyfrom=E2Ctranslationproblem of thrombosis formation of artificial blood vessels in clinical application has become the focus of attention. Some researchers have studied the preparation of artificial blood vessels with synthetic polymer materials, but they are easy to thrombosis when used for small caliber (≤6 mm) vascular grafts. Silk fibroin is a natural biological material, has better biocompatibility compared with synthetic polymer materials. For the application of silk fibroin in artificial blood vessels, the anticoagulant property of the regenerated silk fibroin materials and hirudin modified materials were studied.Dacron and polytetrafluoroethene (PTFE) are commonly used for preparating artificial blood vessels. In the study, Dacron and PTFE were parallel studied as the controls, the anticoagulant property of ethanol treated SF films was studied. Hemolysis rate tests showed that hemolysis rate of ethanol treated SF films was lower than Dacron and PTFE. The SEM /search?q=photographamp;keyfrom=E2Ctranslationphotographs and platelet adhesion ratios showed that platelet adhesion ratios of ethanol treated SF films were significantly less than that of the control samples, the platelet activation measures also proved this conclusion; recalcification time assay showed time of ethanol treated SF films was slightly lower than that of Dacron and PTFE.The use of crosslinking agent is a kind of important method for preparating water insoluble regenerated silk fibroin material. Polyethylene glycol diglycidyl ether (PEG-DE) is a flexible polymer and can improve the flexibility of regenerated silk fibroin material, the anticoagulant property of PEG-DE crosslinked SF materials was studied. Hemolysis rate tests showed that hemolysis rate of PEG-DE crosslinked SF films was slightly higher than that of et