DNA甲基化研究方法的重大突破(原文).pdfVIP

Downloaded from on November 21, 2012 - Published by Cold Spring Harbor Laboratory Press Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing Andrew Adey and Jay Shendure Genome Res. 2012 22: 1139-1143 originally published online March 30, 2012 Access the most recent version at doi: 10.1101/gr.136242.111 Supplemental /content/suppl/2012/03/30/gr.136242.111.DC1.html Material References This article cites 15 articles, 5 of which can be accessed free at: /content/22/6/1139.full.html#ref-list-1 Creative This article is distributed exclusively by Cold Spring Harbor Laboratory Press Commons for the first six months after the full-issue publication date (see License /site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at /licenses/by-nc/3.0/. Email alerting Receive free email alerts when new articles cite this article - sign up in the box at the service top right corner of the article or click here To subscribe to Genome Research go to: /subscriptions © 2012, Published by Cold Spring Harbor Laboratory Press Downloaded from on November 21, 2012 - Published by Cold Spring Harbor Laboratory Press Method Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing Andrew Adey and Jay Shendure1 Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA We have adapted transposase-based in vitro shotgun library construction (‘‘tagmentation’’) for whole-genome bisulfite sequencing. This method, Tn5mC-seq, enables a 100-fold reduction in starting material relative to conventional