猪圆环病毒2型灭活疫苗(SH)株间接ELISA方法的建立.docVIP

目 录 题目……………………………………………………………………………1 摘要……………………………………………………………………………1 关键词……………………………………………………………………………1 前言……………………………………………………………………………2……………………………………………………………………2 2 实验原理………………………………………………………………………3 3 小鼠免疫实验………………………………………………………………………3 4 ELISA检测方法……………………………………………………………………3 5．实验数据1………………………………………………………………………….4 5.1实验数据2…………………………………………………………………………5 5.2实验数据3………………………………………………………………………...6 6.结果分析………………………………………………………………………….....6 7.小结………………………………………………………………………………….6 参考文献………………………………………………………………………………6 致谢……………………………………………………………………………………猪圆环病毒2型灭活疫苗（SH）株间接ELISA方法的建立 摘 要：准备了3批不同抗原含量的猪圆环病毒2型（PCV2）灭活疫苗，分别为PCPCPCV211O2O13。对此3批PCV2灭活疫苗，利用本实验室建立的小白鼠效力检验标准进行效力检验，即以PCV2参考疫苗作为对照品，测定待检疫苗免疫小白鼠后血清中PCV2抗体效价的高低来判定疫苗效力。3次重复检测结果都显示对照组应全部为阴性，待检疫苗免疫组平均抗体效价不低于参考疫苗免疫组平均抗体效价，参考疫苗免疫组平均抗体效价不低于1:800。表明这3批圆环疫苗灭活合格。这与猪体内的效力检验结果一致，表明建立的针对猪圆环病毒2型灭活疫苗的小白鼠效力检验方法具有良好的准确性和重复性。 关键词：圆环病毒型；灭活疫苗；效力检验；抗体检测 Abstract: Prepared three batches of the different content of antigen pig wreath virus type 2 (PCV2) inactivated vaccine， respectively PC PC PCV211O2O13. This PCV2 three batches of inactivated vaccine， with the establishment of the laboratory mice inspection standard inspection effect play， namely to PCV2 reference vaccine as reference substance， vaccine immune mice were waiting in the serum PCV2 antibody titer level to determine the vaccine effect. 3 times repeat testing results have shown that the control group should all be negative， inspected the vaccine immune group is not lower than average antibody titer reference vaccine immune group average antibody titer， reference vaccine immune group average antibody titer was not under 1:8 00. Shows that the three rings inactivated vaccine of qualified. The pigs and the validity of the test results in the body consistent， show that the established for pig wreath virus type 2 inactivated vaccine little mice effect inspection method has good repeatability and accuracy. Key words: Wreath virus type; In