高效液相色谱法测定护肝胶囊中五味子醇甲的含量_论文.docxVIP

高效液相色谱法测定护肝胶囊中五味子醇甲的含量 【摘要】 目的探讨大蒜素对血液流变性及红细胞生理特性的影响。 方法制作急性高黏滞大鼠模型动脉取血，通过黏度法测定血流变指标及红细胞聚集指数，核孔膜滤过法测定红细胞滤过指数，荧光偏振法测定红细胞膜流动性，以及红细胞渗透脆性试验，比较大蒜素腹腔注射组和未处理组的差异。 结果与未处理组比较，大蒜素高、中、低剂量组对血瘀症大鼠全血黏度的增加均有明显降低作用（P＜，P＜）；中、高剂量组对血浆黏度有明显降低作用（P＜），且上述作用呈现一定的量效关系；高剂量组可明显降低红细胞压积（P＜），可明显提高红细胞的变形性（P＜）；中、低剂量组有降低红细胞聚集性的作用；各剂量组对红细胞渗透脆性的增大有改善作用；各剂量组对红细胞膜流动性的降低有改善作用，但均不具有统计学意义。结论 大蒜素可提高红细胞变形性和降低血液黏度，改善血液流变性，而用于血淤症的防治。 【关键词】 大蒜素； 急性血淤症； 血液流变性； 红细胞 Effects of Allicin on Hemorheology and Physiological Properties of Erythrocytes in Acute Blood-stagnated Rats Abstract:ObjectiveTo explore the effects of allicin on hemorheology and physiological properties of established the rat models of acute hyperviscosity and collected artery blood samples. Then we examined the hemorheology index and erythrocyte aggregation index by the viscosity methods, detected the erythrocyte filtration index by the nuclear pore film, determined erythrocyte membrane fluidity by the fluorescence polarization and performed erythrocyte osmotic fragility test. The difference between the allicin intrapertoneal injection group and the untreatment group was comparison with the untreatment group, all the high-, medium- and low-dose groups of allicin treatment had a decline in the enhancement of whole blood viscosity in rats with blood stasis (P＜, P＜). In the high- and medium-dose groups, the plasma viscosity significantly decreased (P＜) and had the dose-effect relationship. In the high-dose group, the hematocrit greatly decreased (P＜) and the erythrocyte deformability increased (P＜). In the medium- and low-dose groups, the erythrocyte aggregation index decreased. All dose of allicin had effect on the increase of erythrocyte osmotic fragility and the decrease of erythrocyte membrane fluidity, but no significance was detected. ConclusionAllicin may increase the erythrocyte deformability, decrease the whole blood viscosity and improve the blood rheological properties and it can be used in the treatment of blood sta