b7h1在胃癌组织中的表达及临床意义word格式论文.docxVIP

B7- H1 在胃癌组织中的表达及临床意义中文摘要目的：研究人胃癌组织中 B7-H1 的表达水平，并探讨其与年龄、性别、肿瘤部位、肿 瘤大小、浸润深度、分化程度、有无远处转移等临床病理指标的相关性。 方法：共取临床标本 45 例。其中 15 例标本分别取胃癌组织及癌旁正常组织， western blot 观察胃癌组织及癌旁正常组织中 B7-H1 的表达情况；同时使用流式细胞仪 检测胃癌及癌旁组织中巨噬细胞表面 B7-H1 分子的表达情况。余下 30 例仅取 胃癌组织通过流式细胞仪检测 B7-H1 的表达量，联合临床病理资料，分析 B7-H1 与临床指标的相关性。 结果：1.western blot 显示在胃癌患者中，均可检测到 B7-H1 分子且胃癌组织比癌旁 正常组织中 b7-H1 的表达水平高。 2.流式细胞仪检测得知胃癌组织及癌旁正常组织中巨噬细胞都有 B7-H1 的表 达，胃癌组织 B7-H1 表达比癌旁组织高，比例分别为 20.71 ± 3.69%和 6.83 ± 1.80% （P0.01）。 3 经流式检测结果：B7-H1 的高表达与患者的性别、年龄、肿瘤发生部位、肿 瘤大小、浸润深度、分化程度无关（P0.05）;而与患者是否有远处转移相关（P0.05）. 结论：1.B7-H1 可能会成为判断患者预后的一个新指标，临床上通过 B7-H1 检测，可 对具有高风险预后较差的病人尽早实施有效的治疗措施来防止疾病的恶化。 2.使用 B7-H1 拮抗剂可以阻断 B7-H1/PD-1 通路传递的抑制信号，可能成为胃 癌免疫治疗新靶点。 主题词：B7-H1胃癌 流式细胞仪 The expression of B7-H1 in gastric carcinoma and its clinical significanceAbstract Objective: To investigate the level of B7-H1 expression in the human gastric cancer, and its correlationship with age, sex, location and the clinical pathological indicators such as the size, location, depth of invasion, differentiation and distant metastasis of tumor.Methods: A total of 45 clinical samples were obtained for investigation. In 15 of them samples were taken from the gastric carcinoma and its adjacent normal tissue respectively for each one. Western Blot was used to investigate the expression of B7-H1 in gastric carcinoma and its adjacent normal tissue, in which the expression of B7 -H1 molecule on the surface of macrophages was measured using flow cytometry as well. The other 30 samples were only taken from gastric cancer and the expression of B7-H1 molecule on the macrophages were studied by flow cytometry. Then, the results obtained were used to analyze the correlationship between the expression of B7-H1 and clinical indicators in association with clinical pathological data.Results: 1.The data of Western Blot indicated that B7-H1 molecule can be detected in all of the patients with gastric carcinoma and its expression level is significantly higher in gastric carcinoma than in its