cski在动脉粥样硬化中的作用 机制与调控分析word格式论文.docx

缩略语表英文缩写α-SMA英文全称α-smoothmuscleactin中文全称α-平滑肌细胞actinAdAdenovirus腺病毒ASAtherosclerosis动脉粥样硬化β-galβ-galactosidaseβ-半乳糖苷酶bpBasepair碱基对BSABovineserumalbumin牛血清白蛋白ChIPChromatinimmunoprecipitation染色质免疫共沉淀cDNAComplementaryDNA互补DNADBDDNA-bindingdomainDNA结合域DEPCDiethylPyrocarbonate焦碳酸二乙酯DMSODimethylSulfoxide二甲基亚砜DNADeoxyribnucleicacid脱氧核糖核酸dNTPDeoxyribonucleosidetriphosphate脱氧核糖核苷三磷酸DTTDithiothreitol二硫苏糖醇EMSAElectrophoresismobilityshiftassay凝胶迁移滞后实验E.coliEscherichiacoli大肠杆菌EDTAEthylenediaminetetraaceticacid乙二胺四乙酸EPEppendorf离心管HEHematoxylin-eosinstaining苏木精-伊红染色法Hourh小时HRPHorseradishperoxidase辣根过氧化物酶LBDLigand-bindingdomain配体结合域LucLuciferase荧光素酶MAPKM-MLVRTMitogen-activatedproteinkinasesMoloneyMurineLeukemiaVirus促分裂素原活化蛋白激酶莫洛尼(氏)鼠白血病病毒ReverseTranscriptase反转录酶MCSMultipleclonesite多克隆位点minMinute分钟miRNA mRNAncRNA ntOD ORF PPARγPBS PMSF PVDF RISC RNAirpmRT-PCRRXR snRNAU6 SDSmicroRNA MessengerRNA Non-codingRNA NucleotidesOpticaldensityOpenreadingframePeroxisomeproliferatorsactivatedreceptorsγPhosphatebufferedsalinePhenylmethylsulfonylfluoridePolyvinylidenefluorideRNA-incucedsilencingcomplexRNA interferenceRevolutionsperminuteReversetransciptase-PolymerasechainreactionRetinoidXreceptorSmallnuclearRNAU6Sodiumdodecylsubphatepolyacrylamidegelelectrophoresis微小RNA信使RNA 非编码RNA 核苷酸光密度开放读码框过氧化物酶体增殖物激活受体γ磷酸盐缓冲液苯甲基磺酰氟聚偏氟乙烯RNA诱导的基因沉默复合物RNA干扰每min转数反转录多聚酶链式反应视黄醇X受体小核RNAU6十二烷基硫酸钠-聚丙烯酰胺凝胶电泳SecondSEMsStandardErrorofMean秒标准误TAETBETBSTGF-βTEMEDU3′-UTR5′-UTRVSMC WBTris-acetate-EDTA(buffer)Tris-borate-EDTAbuffer Tris-bufferedsalinetransforminggrowthfactorβN,N,N’,N’-tetramethylethylenediamineUnit3′-Untranslatedregion5′-UntranslatedregionVascularsmoothmusclecellWesternblotTris/乙酸/EDTA缓冲液Tris/硼酸/EDTA缓冲液Tris盐缓冲液转化生长因子βN,N,N’,N’-四甲基乙二胺单位3′ 端非翻译区5′ 端非翻译区血管平滑肌细胞免疫印迹试验Effect,mechanismandregulationofc-SkiinatherosclerosisAbstractAtherosclerosis(AS)isaconditionthatanarterywallthickensandmultipleplaques form withinthearterieswhichareresultedfromtheaccumulationoffattymaterialssuchas cholesterolandtriglyceride.Thiscau