c肽在胰岛素诱导血管平滑肌细胞增生和迁移中作用word格式论文.docxVIP

C 肽在胰岛素诱导的血管平滑肌细胞增生和迁移中的作用余小平，尤文俊（导师） 中文摘要目的 ：探讨C-肽对胰岛素诱导的人脐血管平滑肌细胞(vascular smoothmuscle cells,VSMCs)增殖与迁移的影响及可能机制。方法 ： 培养人脐血管平滑肌细胞，采用四甲基偶氮唑盐（MTT）比色法测定 VSMCs增殖; Boyden小室法检测VSMCs 的迁移；采用Western blot 检测磷酸化 蛋白激酶B(p-Akt) 和磷酸化细胞外信号调节激酶( p-ERK) 水平的变化。结果 ： 胰岛素促进血管平滑肌细胞的增生与迁移；C-肽能够有效抑制胰岛素诱 导的血管平滑肌细胞的增生与迁移；C-肽联合胰岛素能够明显抑制ERK、Akt的 磷酸化水平。结论： C-肽能够抑制胰岛素诱导的血管平滑肌细胞的增殖与迁移，联合应用C- 肽对于胰岛素治疗患者的血管并发症可能有利，且C-肽抑制胰岛素诱导的人脐血 管平滑肌细胞增殖可能的分子靶点是Ras-Raf-ERK/MAPK和Ras-PI3K-Akt/PKB 信号传导通路。关键词：血管平滑肌细胞 胰岛素 C-肽 细胞外信号调节激酶 蛋白激酶 B2The mechanism and effect of C-peptide on the proliferation and migration of umbilical vascular smooth muscle cells induced by insulin.Yu Xiaoping, YOU WenjunAbstractObjective: To investigate the possible mechanism and effect of C-peptide on the proliferation and migration of umbilical vascular smooth muscle cells induced by insulin.Methods: (methyl thiazolyl tetrazolium,MTT)MTT test was applied to determine the inhibition ratio and the relationship between the duration MTT chromatometry test was applied to determine the proliferation of VSMCs; Boyden chambers were used to observe the migration of VSMCs；By using Western blot ,the phosphorylation status of Akt and ERK was detected.Results: Insulin augmented VSMCs proliferation and migration ;effects were abrogated by C-peptide . C-peptide affect insulin-induced Akt or extracellular signal-regulated kinase signaling.Conclusion: Our data support a causative role for insulin in VSMCs with a novel counter-regulatory effect of proinsulin C-peptide.Thus ,C-peptide can limit the detrimental effects of insulin on VSMCs function.Co-supplementing insulin therapy with C-peptide could improve therapy in insulin-treated patients. C-peptide ablates the effects of insulin on VSMCs maybe acting by inhibiting the ERK or PI3K/Akr pathways, the two major pathways that lie downstream of the insulin receptor.3Key words ： vascular smooth muscle cellinsulinc-peptide proliferationmigrationExtracellular signal-regulated kinaseprotein kinase B4前 言随着我国