dccik细胞与顺铂对乳腺癌细胞mcf7杀伤作用的分析word格式论文.docxVIP

AbstractObjective：This studywas aimed toinvestigate thein vitro inhibitoryefects of DC(dendritic cel1)-CIK (cytokine-induced killer cel1)cocultured cells combinedwith cis-platinum againstBreast cancer cell lineMCF-7cells.Methods：Inthis study，the DC cells andCIK cells co-cultured DC-CIK cells andinMCF-7 cells co-culturedfor12，24 and 48 hours，usingthe MTTassayMCF-7 cell proliferation inhibition and usingdifferentconcentrations the chemotherapydrug cisplatinrolein MCF-7 cells，usingthe sameMTTassayMCF-7 cell proliferationinhibition.Thencomparethe inhibitoryeffect of DC-CIKcells andthe chemotherapydrugcisplatin proliferation ofMCF-7 cells.Results：Addition ofDC-CIK cells usingMTTassayDC-CIK role in theMCF-7 cellproliferation ofMCF-7 beforeand after theshow，regardless of the role of 12，24 or48 hours，MCF-7 cells apoptosis，proliferation wassignificantlyinhibited. DC-CIKand cisplatin on MCF-7 proliferation inhibition comparison，5×104cells proliferation ofMCF-7cells 12h inhibition with 40ng/ml concentration of cisplatin on theproliferation ofMCF-7 cells for12h inhibition equivalent for24h inhibited with 50 ng/ml cisplatinequivalent to the inhibition ofproliferation ofMCF-7 cells 24h，48h role in the inhibitionof60ng/ml of cisplatin on the proliferation ofMCF-7 cells the 24h ofinhibition basic equivalent. Theresults show that the DC-CIK cells to inhibit theproliferation ofMCF-7 cells.Conclusion：This studyDC-CIK therapyforbreast cancer madealaboratoryforbasic research，the results show that this approach can achievethe same chemotherapy drugs inhibit the proliferation ofbreast cancer cells.Theregardless of the role of12，24 or 48 hours，MCF-7cells apoptosis，proliferation was significantlyinhibited. DC-CIKand cisplatin on MCF-7 proliferation inhibition comparison，5×104cells proliferation ofMCF-7 cells 12h inhibition with 40ng/ml concentration of cisplatin on theproliferation ofMCF-7 cells for12h inhibition equivalent for24h inhibited with 50ng/ml cisplatin equivalent to the inhibitio