dsc2在食管癌中的表达 功能与分子调控机制的分析word格式论文.docxVIP

优秀毕业论文 精品参考文献资料 ABSTRACT Desmocollin 2 (DSC2) is a desmocollin subfamily member of the cadherin superfamily involved in desmosomal cell-cell adhesion and plays a critical role in the maintenance of normal tissue architectures in the epithelium. It was recognized recently that changes in intercellular adhesion were accompanied by tumor dedifferentiation, progression and reduced expression of several desmosomal cadherin molecules. However, reports exploring the link of DSC2 expression to esophageal squamous cell carcinoma (ESCC) are few. Our recent research work using cDNA microarray, migration and adhesion analysis performed on a fascin RNAi-depleted ESCC cell line showed that down-regulation of fascin resulted in suppression of cell migration and promotion in cell adhesion along with significant up-regulation of DSC2 expression, indicating that DSC2 might be involved in ESCC development and progression. In the present study on the role of DSC2 in esophageal cancer progression, we address three fundamental questions: (a) What is the expression pattern of DSC2 in ESCC? To which extent the pattern of expression is correlated with clinical outcomes of patients with ESCC? (b) Does DSC2 affect tumor cell behaviors and how does it reduce the aggressiveness of tumor cells? (c) What is/are the mechanism/s that control DSC2 expression in ESCC cells? In this study, DSC2 expression was first evaluated in ESCC specimen using real-time PCR, immunohistochemistry and western blotting. The results showed that DSC2 expression in ESCCs was decreased significantly both in protein and mRNA levels and this reduction was correlated with cell differentiation (P=0.002), pTNM stages (P=0.036) as well as poor survival for the patients (P=0.029). In addition to DSC2, the expression levels of other desmocollin subfamily members such as DSC1 and DSC3 were also measured through semi-quantitative RT-PCR. It is noteworthy that DSC3 exhibited same expression levels in ESCC tumors and matched norm