egr1在正常卵巢及卵巢良 恶性肿瘤组织中的表达及其临床意义word格式论文.docx

优秀毕业论文 精品参考文献资料 ABSTRACT Objective: To investigate the expression of EGR-1 in normal ovarian tissues,benign ovarian tumour and malignant tumour,and to explore the correlation of EGR-1 with occurrence, development clinical significance in ovarian cancer. Method： A total of 20 samples of normal ovarian tissue, 20 samples of benign tumors and 60 cases of malignant tumors were collected.To choose these subjects based on postoperative pathological results of the first affiliated hospital of nanchang university and without any preoperative treatment. The expression of EGR-1 mRNA was detected by Real-time fluorescent quantitative PCR respectively in all collecting cases. The expression of EGR-1 protein was detected by immunohistochemical method respectively in all collecting cases. Follow up all the data of these subjects ,included age,tumor type,lymphatic metastasis,tumorous grading and staging through telephone. Results: The expression of EGR-1 mRNA The expression of EGR-1 mRNA ,both in normal ovarian tissues （2.98??0.24） and benign ovarian tumour （2.87±0.20）were significant higher than ovarian cancer （1.02±0.16）（P 均＜0.05）. There were no significant differences between normal ovarian tissues and benign ovarian tumour（P ＞0.05）. The expression of EGR-1 protein Immunohistochemistry showed that positive expression of EGR-1 protein distribution in the nucleus,only a little expressed in cell cytoplasm.The expression of EGR-1 protein was judged by the cell staining intensity and positive percentage .The expression of EGR-1 protein,both in normal ovarian tissues （7.41±0.97）and benign  PAGE 5 ovarian tumour （6.94±1.07）were significant higher than ovarian cancer（4.08±0.63） （P 均＜0.05）. There were no significant differences between normal ovarian tissues and benign ovarian tumour（P ＞0.05）. The relationship between the expression of EGR-1 and the patients with ovarian cancer clinical indicators Using statistical methods shows that the low expression of EGR-1 protein in ovarian c