egfr在卵巢上皮性肿瘤中的表达及rnai靶向作用的分析word格式论文.docx

Abstractproliferation, cell growth specificity transfection group was obviously lower blank plasmid group and the control group, cell inhibition rate was 61.8%. Tablet colony experiment, specific group transfection cell colony formation inhibition rate was 59.9%.Conclution:Border EGFR protein in ovarian tumors and ovarian epithelial carcinoma has expressed, and gradually increased. Prompt EGFR gene may be a important factor in the development of ovarian epithelial tumor formation.Targeting the EGFR gene RNA interference can reduce the expression of EGFR, SKOV3 cells leads to cell death, reducing cell proliferation. So the targeting EGFR RNA interference technology used in ovarian epithelial tumor target gene therapy research has certain feasibility.Keywords ： EGFR ； Ovarian epithelial tumors ； RNA interference ； Genetic mutations；SKOV3cell line目 录第 1 章 引 言....................................................................................................... 1第 2 章 材料与方法............................................................................................. 32.1 材料......................................................................................................... 32.2 实验方法................................................................................................. 52.3 统计学处理............................................................................................ 11第 3 章 结果....................................................................................................... 12 3.1 EGFR 在卵巢上皮性肿瘤中的表达..................................................... 123.2 RNA 干扰 EGFR 对 SKOV3 细胞的作用 143.2.1 EGFR 表达情况 143.2.2 MTT 法检查细胞增殖情况 153.2.3 集落形成实验............................................................................. 153.2.4 RT-PCR 检测 16第 4 章 讨论....................................................................................................... 17 4.1 EGFR 蛋白在卵巢上皮性肿瘤中的表达............................................. 174.2 靶向 EGFR 基因 RNA 干扰对卵巢上皮性肿瘤的影响