erk12pparαscad信号途径对生理性和病理性心肌细胞肥大的调控研究word格式论文.docx

目录中文摘要·······························································································IAbstract······························································································III第一章引言·························································································11.1 心肌肥大的分类·················································································11.2 心肌肥大能量代谢变化········································································21.3 ERK1/2 的研究现状及其与心肌肥大的关系31.4 PPARα 的研究现状及其与心肌肥大的关系41.5 SCAD 的研究现状及其与心肌肥大的关系41.6结语································································································51.7研究思路··························································································5第二章生理性和病理性心肌细胞肥大的比较·················································62.1前言································································································62.2实验材料··························································································72.2.1 实验动物·····················································································72.2. 2主要药物与试剂············································································72.2. 3 主要实验仪器和器材······································································82.2. 4主要试剂配置···············································································92.3实验方法························································································102.3.1心肌细胞原代培养········································································102.3.2心肌细胞表面积测定······································································11心肌细胞总RNA提取以及Real-time PCR11心肌细胞总蛋白提取以及Western blot 检测142.3.5 心肌细胞SCAD 酶活性测定162.3.6心肌细胞游离脂肪酸含量测定·······················································