黄花苜蓿组织培养再生体系建立及品质改良转基因分析word格式论文.docxVIP

优秀毕业论文 精品参考文献资料 Establishment of Tissue Culture Regeneration System and Transgene for Quality Improvement in Medicago falcata L. Abstract Alfalfa was important legume forages, and its resources was very rich in our country, but the digestion rate was low, so how to improve the digestion rate and quality, enhance the nutritional value were the main tasks of workers for the alfalfa breeding. With the development of genetic engineering, molecular method had been used widely of alfalfa breeding, it made breeding process speed up after clearing the breeding targets. In this study, it was as materials that optimization for aseptic culture, tissue culture regeneration system and Agrobacterium-mediated genetic transformation system had been established, and aimed to obtain higher sulfur amino acids and lower lignin of transgenic plants of Alfalfa by transgenic means. The results as followed: The best growth conditions for Alfalfa was seed pretreatment about 70s by 75﹪ alcohol, 0.1﹪HgCl2 and concussion disinfection 8min in add 30 g??L-1 sucrose in MS culture. The germination rate could reached 91.32﹪; 0.1﹪ HgCl2 sterilization time was key factors affecting the growth of seedlings, so did sugar concentration. The results that regeneration system of native Alfalfa. showed that the callus induction rate could be up to 100﹪ of cotyledons and hypocotyls were cultivated for 10d on the optimal medium for callus induction that MS +1.5 mg·L-1 2,4-D + 0.4 mg·L-1 6-BA + 3﹪sucrose + 0.7﹪ agar. The optimal medium for embryogenic callus was MS + 0.5 mg·L-1 KT + 0.1 mg·L-1 NAA + 2﹪sucrose + 0.7﹪agar, and the rate of embryogenic callus was 81.5﹪.The optimal medium for differentiated buds was MS + 0.25 mg·L-1 KT+0.05 mg·L-1 NAA+2﹪sucrose+0.7﹪agar, the rate of differentiated buds was 36.5﹪. The optimal rooting medium was 1/2MS + 0.5 mg·L-1 NAA + 1.5﹪sucrose +0.7﹪agar, and the rooting rate was 93.3﹪. A third of the plant’s callus was better than other plants’ on the same hormone level. There w