IGFBP5及cFLIP在宫颈上皮内瘤变及宫颈癌组织中的表达及意义.docVIP

IGFBP5及cFLIP在宫颈上皮内瘤变及宫颈癌组织中的表达及意义 作者：侯雪晶 单位：山东大学齐鲁医院妇产科, 济南 【摘要】 目的 研究胰岛素样生长因子结合蛋白5 (IGFBP5)、细胞型Fas相关死亡结构域蛋白样白介素1beta;转换酶抑制蛋白（cFLIP）及高危型人乳头瘤病毒(HPV)在宫颈癌和宫颈上皮内瘤变(CIN)组织中的表达，并探讨其临床意义。方法 采用RTPCR及免疫组织化学SP法检测28例正常宫颈组织、37例CIN、40例宫颈癌组织中IGFBP5、cFLIP表达，二代杂交捕获法检测上述患者宫颈分泌物中高危型HPV DNA。结果 IGFBP5蛋白在正常组织、CIN、宫颈癌中表达差异有统计学意义（Plt;0.05），在CINⅡ/Ⅲ组达高峰，在宫颈浸润癌中IGFBP5表达下降，且与临床分期、病理分化程度及淋巴结转移有关。IGFBP5 mRNA在宫颈癌中高于正常组织，但低于CIN组（Plt;0.05）。cFLIP蛋白及mRNA在正常组织未见表达,在CIN及宫颈癌中随病变程度加深而表达量增加，各组表达差异有统计学意义(Plt;0.05)。HPV阳性率在CIN及宫颈癌中高于正常组（Plt;0.05）。结论 IGFBP5及cFLIP与宫颈病变的发展有关，可用于判断患者病情变化及预后。 【关键词】 胰岛素样生长因子结合蛋白5 细胞型Fas相关死亡结构域蛋白样白介素1beta;转换酶抑制蛋白 宫颈肿瘤 宫颈上皮内瘤样病变 cervical carcinoma and their clinical significances HOU Xuejing, LIU Xin, QIAO Yunbo, ZHANG Youzhong (Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China) To investigate the expression of insulinlike growth binding protein 5 (IGFBP5), cellular fasassociated death domainlike interleukin1beta;converting enzymelike inhibitory protein (cFLIP), and high riskHPV in cervical intraepithelial neoplasia（CIN）and cervical carcinoma（CC）and to evaluate their clinical significances. Methods Immunohistochemistry (SP) was used to determine the expressions of IGFBP5 and cFLIP proteins in 28 normal cervical tissues, 37 CIN and 40 CC tissues, RT PCR was used to determine the mRNA, and hybrid capture Ⅱ assay was used to determine the highrisk HPV DNA. Results IGFBP5 protein significantly expressed in normal tissues, CIN and CC tissues, and it was the highest in CINⅡ/Ⅲ but it was decreased in CC tissues (Plt;0.05). IGFBP5 protein expression had relationships with the clinical stage, cell differentiation and lymph node metastasis. However, IGFBP5 mRNA expression was higher in the CC group than that in the control group but was lower than that in the CINⅡ/Ⅲ groups. cFLIP expression was not found in normal cervical tissues, but was gradually increased in the CIN and CC groups with an increase of patholo