凋亡抑制蛋白survivin与子宫腺肌瘤关系的研究.docVIP

凋亡抑制蛋白survivin与子宫腺肌瘤关系的研究 【摘要】 目的探讨凋亡抑制蛋白survivin在子宫腺肌瘤在位内膜、异位内膜的表达，研究以survivin为核心的细胞凋亡、增殖及血管生成机制与子宫腺肌瘤发病的相关性。方法采用免疫组化法检测子宫腺肌瘤在位内膜、异位内膜及正常对照组内膜中survivin, PCNA, endoglin蛋白表达。结果子宫腺肌瘤异位内膜组survivin蛋白的表达和微血管密度显著高于在位内膜组和异位内膜组（P0.01）,增殖期宫内膜、分泌期宫内膜survivin蛋白表达与endoglin蛋白表达呈显著相关性。结论survivin的过度表达可能参与了子宫腺肌瘤的发病过程，以其为核心的细胞凋亡和血管生成机制与子宫腺肌瘤的发病关系密切。 【关键词】 子宫腺肌瘤；survivin；凋亡抑制蛋白；血管生成 The Relativity of survivin and Adenomyosis LIU Ping, LUO Xin*, SHI Yuxia Department of Obstetrics and Gynaecology, Renmin Hospital, Wuhan University,Wuhan 430060, China;Department of Obstetrics and gynaecology,The First Affiliated Hospital of Jinan University (*Corresponding Author) Abstract：Objective To investigate the expression of survivin in normal human endometrium, and adenomyosis in order to study the function and relativity of apoptosis, cell proliferation and angiogenesis in the course of adenomyosis.Methods Specimens of normal endometrium, ectopic endometrial tissue and eutopic endometrial tissue in adenomyosis were obtained for immunohistochemical survivin, PCNA and endoglin analyses.Results The expression of survivin and microvessel density in ectopic endometrial tissue of adenomyosis was significantly higher than that of normal endometrium and eutopic endometrial tissue in adenomyosis (P0.01), survivin expression was significantly associated with endoglin expression in endometrium of proliferate phase and secretary phase（r=0.612,P＜0.01）.Conclusion The overexpression of survivin and survivincollected apoptosis and angiogenesis mechanism might play a pathogenic role in the development of adenomyosis. Key words：Adenomyosis; survivin; Inhibitor of apoptosis protein; Angiogenesis 0 引言 子宫内膜异位性疾病是一种良性疾病，但具有局部播散、远处转移的恶性行为。子宫腺肌瘤是内膜异位性疾病之一，是指具有生长功能的子宫内膜和间质侵入子宫肌层，但其病因至今不明。近年来研究表明，细胞凋亡及血管生成可能在子宫内膜异位症及子宫腺肌瘤的发病过程中起重要作用。survivin（SVV）作为凋亡抑制蛋白（inhibitor of apoptosis protein, IAP）家族的一个新成员，越来越受到学者们的重视。SVV选择性的表达于常见恶性肿瘤，而在癌旁正常组织和成人分化组织中不表达，是一个肿瘤特异性的凋亡抑制因子。国外对于SVV与良性疾病的相关性研究较少，而SVV与子宫腺肌瘤关系的研究尚未见报道。本研究探讨SVV蛋白在