天狼猩红―偏振光法检测子宫内膜异位症中Ⅰ，Ⅲ型胶原.docVIP

天狼猩红―偏振光法检测子宫内膜异位症中Ⅰ，Ⅲ型胶原 【关键词】 子宫内膜异位症 关键词: 子宫内膜异位症;胶原;荧光偏振;天狼猩红 摘 要:目的 探讨子宫内膜异位症中Ⅰ，Ⅲ型胶原的含量和作用. 方法 对20例子宫内膜异位症（EM）患者的异位内膜组织（红色病变组和黑色病变组）和11例非子宫内膜异位症患者的在位内膜组织（对照组）用天狼猩红染色，偏振光观察，photoshop分析，半定量研究Ⅰ，Ⅲ型胶原的含量. 结果 Ⅰ，Ⅲ型胶原在对照组中含量最低（P0.05，P0.05），Ⅲ胶原在红色病变组中含量最高（P0.05），Ⅰ型胶原在黑色病变组中含量最高（P0.05）.Ⅰ，Ⅲ型胶原在增生期和分泌期无明显差异（P0.05）. 结论 子宫内膜异位症患者的异位组织中有Ⅰ，Ⅲ型胶原的表达.Ⅰ，Ⅲ型胶原的异常表达可能在EM的发病机制和病程进展方面有重要作用. Picrosirius┐polarization determination of typeⅠ，Ⅲcollagen in endometriosis implant LI Jie ，XIN Xiao-Yan ，CUI Geng 1 Department of Gynecology Obstetrics，2 Department of Orthopedics，Xijing Hospital，Fourth Military Medical University，Xian710033，China Keywords:endometriosis;collagen;fluorescence polariza-tion;sirius red Abstract:AIM To determine typeⅠ，Ⅲcollagen present in ectopic endometrium and in the connective tissue surround-ing ectopic endometrial implant，and to valuate the degree of fibrosis in the endometriosis.METHODS Normal eutopic endometrium，red lesion and black lesion were collected and typeⅠ，Ⅲcollagen detected by sirius red and polarization microscopy.The percentage of the stained area in the tissue was measured with a computerized image analysis system.RESULTS There was significant difference in the mean per-centage of the total stained area among the three groups（A，B，C）.Amount ofⅠ，Ⅲtype collagen was the lowest in group C（P0.05，P0.05），and the stained area of type-Ⅲcollagen in group A was the highest among the three groups（P0.05）.The amount of type-Ⅰcollagen was sig-nificantly higher in group B than in group A and group C（P0.05）.CONCLUSION This study demonstrates the presence of typeⅠ，Ⅲcollagen in the intrauterine and ec-topic endometrium of patient with endometriosis.And typeⅠ，Ⅲcollagens play important roles in occurrence and devel-opment of endometriosis. 0 引言 研究表明，约有10%的育龄妇女患有子宫内膜异位症（Endometriosis，EM）［1］ ，且近些年来有逐渐升高的趋势.目前为多数研究者所接受的是1927年Sampson［2］ 提出的经血逆流种植理论，根据这一理论，在子宫内膜种植和增生之前，它们必须到达盆腔，粘附在腹腔间皮的表面.为了种植和生长，子宫内膜细胞必须与腹膜建立细胞-细胞或细胞-胞外基质之间的相互作用.近来有证据表明，EM早期损伤中最初脱落的细胞可侵入腹膜的胞外基质