失血性休克大鼠卵巢功能和结构的变化.docVIP

失血性休克大鼠卵巢功能和结构的变化 【关键词】 失血 摘要：目的: 探讨大鼠失血性休克过程卵巢功能和结构的变化。方法： 40只健康SD雌性大鼠随机分为实验1、2、3组（休克1、2、3 h）与对照组，每组10只。采用放射免疫法测定各实验组休克前后及对照组血清促卵泡生成激素（FSH）和雌二醇(E2)的水平，采用免疫组织化学法检测卵巢组织bcl2、 bax 、p53 和fas基因的表达，同时进行光镜观察。结果： 实验组血清FSH水平随休克时间延长依次升高，仅实验3组与对照组相比差异有统计学意义（P0.05）；E2水平随休克时间逐渐降低，实验2、3组与对照组相比差异有统计学意义（P0.01）；实验各组bcl2、 bax 、和fas表达明显上调，与对照组相比差异均有统计学意义（P0.01）；光镜下显示实验组卵巢间质血管扩张、充血和炎细胞浸润，随休克时间延长而进一步加重，实验3组可见到生长卵泡颗粒细胞间间隙增宽，卵泡的边缘皱缩。结论： 急性失血性休克对卵巢功能可造成损害，抑制内分泌功能，也影响生殖细胞的结构和功能，随着休克时间的延长损害加重。 关键词: 失血性休克；卵巢；促卵泡生成激素；雌二醇；凋亡 Effect on ovarian function and structure during acute hemorrhagic shock in rats ZHANG Yingzi, ZHANG Jianlong, MA Qi (Department of Pathophysiology, Xinjiang Medical University, Urumqi 830054, China) Abstract: Objective: To study the effect on ovarian function and structure during hemorrhagic shock of female rats. Methods: 40 rats were randomly divided into control group (n=10), and acute hemorrhagic shock groups (n=30). Experimental model was established by deplete blood from carotid artery keeping the average arterial blood pressure around 40 mmHg and divided into 3 groups of shock 1hour、2hour、3hour. The radioimmunoassay (RIA) was used to determine the serumal levels of follicularstimulating hormone (FSH) and estradiol (E2), Immunohistochemical analysis for apoptotic gene bax、p53、fas and antiapoptotic gene bcl2 expressed in ovary. Alteration of ovarian morphology was also observed under light microscope. Results: The content of FSH increased and E2 decreased in serum（P0.05 or P0.01）; The express of bcl2、bax and fas showed patent positive reaction, p53 had a lower degree, the statistical analysis shown a close correlationship（P0.01）. The dilatation of ovarian interstitial blood vessel and the infiltration of inflammatory cells were showed under the light microscope. Conclusion: There are second ovarian injury during the acute hemorrhagic shock, apoptosis in ovary was induced in the phase of acute hemorrhagic shock, which may play a definite effect in ovarian injury in