基质细胞cav-1表达影响骨髓瘤细胞对蛋白酶体抑制剂的敏感性-the expression of cav - 1 in stromal cells affects the sensitivity of myeloma cells to proteasome inhibitors..docxVIP

基质细胞 Cav-1 表达影响骨髓瘤细胞对蛋白酶体抑制剂的敏感性英文摘要The Cav-1 expression of stromal cells affects the sensitivity of multiple myeloma cells to the proteasome inhibitor AbstractPart I Construction and Identification of Eukaryotic Plasmid Expression Vectors Encoding the shRNA Targeting Human Cav-1 and Its Stable Expression in HUVECObjective: To construct the eukaryotic plasmid vector mediated shRNA interference targeting Cav-1 gene, and transfect it into HUVEC,then screen stably transfected clonal cell line.Methods: Three plasmid expression vector （ pGPHI/Neo-Cav-1-homo 526 、pGPHI/Neo-Cav-1 homo 548、pGPHI/