脊髓背角gabab受体在糖尿病神经痛形成过程中作用-the role of gabab receptor in that formation of diabetic neuralgia.docx

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2018-05-28 发布于上海
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脊髓背角gabab受体在糖尿病神经痛形成过程中作用-the role of gabab receptor in that formation of diabetic neuralgia.docx

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脊髓背角gabab受体在糖尿病神经痛形成过程中作用-the role of gabab receptor in that formation of diabetic neuralgia

RolesofGABABreceptorsinthespinaldorsalhornofratswithdiabeticneuropathicpainABSTRACTObjective:Aswekonw,theneuropathiesareoneofthemostcommonlong-timecomplicationsofdiabetes,andthemorbilityincreseseveryyear.About16%and26%ofdiabeticpatientssufferchronicpain.Thismaybereferredtoasdiabeticneuropathicpainorpainfuldiabeticneuropathy.Themechanismsofdiabeticneuropathicpainareverycomplexandthemanagementisstillquitedifficult.Studiesonthetransmitterphmaraeologyhaveindicatedthatthereexistedchangesofneurotransmittersandreceptorsinspinalcordinducedbyneuropathicpain.ChangesofGABABreceptorscouldaffectthefunctionofGABAergicsystemsothattoaffectthetransmissionandmodulationofnociceptivemessages.ThepersentstudyistoexplorechangesofGABABreceptorsinspinalcorddorsalhornofratswithdiabeticneuropathicpaininducedbySTZ.Characteristicbehavioralchangeswereobservedtoaffecthyperalgesiaofrats,andwealsohadintrathecalinjectedbaclofentoobservethechangeof50%pawwithdrawthreshold(PWT)ofratswithdiabeticneuropathicpain.Methodsofimmunohistochemistry,RT-PCRandwesternblottingwereappliedtoexaminetheexpressionofGABABreceptorsinspinalcorddorsalhorn.StudiesaboutthechangesofGABABreceptorsinspinalcorddorsalhorncouldhelpfindingoutmorespecifictargetsoftreatmentfordiabeticneuropathicpain.Methods:100maleSprague-Dawleyratswererandomlyselectedforthisexperiment,weighing130-150g.Bodyweights,fastingbloodglucoseleveland50%pawwithdrawthresholdofalltheratsweremeasuredbeforeSTZinjection.90ratswereinducedwithasingleintraperitonealinjectionofstreptozocin(50mg/kg)asdiabetes.AtoneweekafterSTZinjection,74ratswhosefastingbloodglucoselevelincreasedupto16.7mmol/Lwereconsidedasdiabeticrats,andtheratiois82%.AttwoweekafterSTZinjection,responsestothemechanicalstimulusofdiabeticratsweremeasuredwithvonFreyfilament,andwhose50%pawwithdrawthreshold(PWT)weredownto4gwereconsidedasdiabeticneuropathicpainmodels.Another10ratswerewereintraperitonealinjectedofsalineascontrols.30diabeticratswererandomlyselectedforbehavioarlexperiment.23didnotappearnerveinju