hnt4基因修饰嗅鞘细胞结合组织工程技术治疗周围神经损伤的分析-analysis of hnt 4 gene modified olfactory ensheathing cells combined with tissue engineering technology in treating peripheral nerve injury.docx

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2018-05-29 发布于上海
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hnt4基因修饰嗅鞘细胞结合组织工程技术治疗周围神经损伤的分析-analysis of hnt 4 gene modified olfactory ensheathing cells combined with tissue engineering technology in treating peripheral nerve injury.docx

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hnt4基因修饰嗅鞘细胞结合组织工程技术治疗周围神经损伤的分析-analysis of hnt 4 gene modified olfactory ensheathing cells combined with tissue engineering technology in treating peripheral nerve injury

chitosantubewasabsorbedwhenratsweresacrificedafter12weeks.(4).Electrophysiologyobservation:comparisonsofNCV,groupAandC,AandDhadsignificantdifferences,butthereweren’tsignificantdifferencesingroupAandgroupB.(5).Histologicalobservation：therecoveryofnerveinHistologicalobservationof12weekswassignificantlybetterthan6weeks.ThenervefiberweretidinessandenlargementwiththesurfacecoveredwiththickermedullarysheathinGroupAandB,butraritaswithlittlecollagentissueandnewbloodvesselsinGroupC,whilemuchwave-shapedfibertissuecanbeobservedinGroupC.ThenervefiberinGroupDweretanglewithlotsofcollagentissue.FluorescencemicroscopeillustratedthatGFPfluorescencewasAgroupexpressedafter12weeks,whichshowedthattheNSCswerestillaliveaftertransplantation.Conclusions:1.ChemicalinhibitorsanddifferentialadherencemethodscanproducelargenumberofOECs,highpurityandlowattachedbyouteratmosphereofcellphysicochemicalpropertyandbiologicalcharacteristics.(2).TheChitosantubesbothhavegoodbiocompatibilityandOECsproliferationinthesurface.Itcanserveasanervebridgeover.(3).UsingChitosantubesassupportingframeandusingOECswhichtransfectedandexpressedNT-4geneasseedscells,toconstructtissueengineeredartificialnerveshasagoodeffectinrepairingnervedefection.Theeffectofitisclosedtoautogenousnervegraft.Soitcouldfurtherraisenerverepairingeffects.Thisstudyhadbroadapplicationprospectsandvalueoffurtherinvestigation.Keywords:OECs;hNT-4Peripheral;Nerveregeneration;ChitosanDirectedby:Prof.ZHAOZhiyingApplicantforMasterdegree:LIZhihui（DepartmentofHumanAnatomyofPreclinicalMedicine)目录1前言.............................................................12试验材料与方法....................................................32.1试验材料......................................................32.2试验方法......................................................53试验结果与分析....................................................103.1体外培养OECs的形态学观察及鉴定103.2OECs转染hNT-4基因过表达慢病毒载体123.3OECs与壳聚糖组织相容性实验的研究123.4臂丛神经离断大鼠模型建立与桥接................................134讨论................................