hsa mir 335与卵巢癌侵袭转移关系的研究-study on the relationship between hsa mir 335 and invasion and metastasis of ovarian cancer.docx

独创性声明本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研 究成果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其 他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集 体，均已在文中以明确方式标明。本人完全意识到本人将承担本声明引起的一切 法律后果。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有 权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和 借阅。本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据 库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。本论文属于保密□ ，在年解密后适用本授权书。不保密□。（请在以上方框内打“√” ）学位论文作者签名：指导教师签名：日期：年月日日期：年月日目录中英文缩略表 ··························································································· 1中文摘要 ··································································································· 4ABSTRACT5前言 ··········································································································· 6材料和方法 ······················································································· 8结果 ··································································································· 27讨论 ··································································································· 36参考文献 ··························································································· 39综述 ··········································································································· 42附录 ··········································································································· 55致谢 ··········································································································· 57英文缩略词表缩略语英文全称中文全称ANOVAAnalysis of variance方差分析APAmmonium persulfate过硫酸铵Bcl-wB-cell lymphoma 2-like 2B细胞淋巴瘤相关蛋白2cDNAComplementary deoxyribonucleic互补脱氧核糖核酸DEPCDiethyl pryocarbonate焦碳酸二乙酯DMSODimethylsulfoxide二甲基亚砜dNTPDeoxyribonucleoside triphosphate三磷酸脱氧核糖核苷HRPHorseradish peroxidase辣根过氧化物酶KDKilodalton千道尔顿MMP-2Matrix metalloproteinase 2基质金属蛋白2ODOptical density吸光度PAGEPolyacrylamide gel electrophoresis聚丙烯酰胺凝胶电泳法PBSPhosphate Buffer Saline磷酸盐缓冲溶液PCRPolymerase chain reaction聚合酶链反应PMSFPhenylmethanesulfonyl fluoride苯甲基磺酰氟化物PVDFPolyv