il17a在铜绿假单胞菌肺部感染小鼠中的表达及其意义-expression and significance of il17a in mice with pulmonary infection caused by pseudomonas aeruginosa.docx

Expression and significance of IL-17A in the lungs of mice infected with Pseudomonas aeruginosaAbstractObjective: To observe Pseudomonas aeruginosa lung infection in a mouse model of IL-17A expression, to analyze the role of IL-17A in Pseudomonas aeruginosa lung infection in mice infected with Pseudomonas aeruginosa lung treatment to find a new way. Methods: Thirty SPF Kunming white female mice, 8-12 weeks old, weighing 17-23 g, were randomly divided into control group and model group (n = 15), model group drops from the mouse trachea into the configured 0.03ml Pseudomonas aeruginosa suspension control group from the mouse tracheal instillation of the same volume of normal saline, the two groups of mice in turns were killed by collected heart blood when the model were builded 3h, 9h, 24h,5 mice each time. Took the lung tissue in mice to do histopathologic slide and observated alveolar inflammation through HE staining, detected expression of IL-6, IL-17A in serum of the mice by ELISA and expression of IL-17A in the lung tissue through immunohistochemical. Results: (1)observed under HE staining light microscopy : the control group: at all time points, the lung tissue structure was clear and the alveolar structure was complete, there was no broken or damaged alveolar walls, the alveolar cavity size and the alveolar interval was normal, also there was no inflammatory cell invasion and fibroustissue hyperplasia . Model group: At the third hour, the lung tissuestructure was clear and the alveolar structure was complete , the alveolar walls were not broken or damaged, and the alveolar cavity size was normal, there was a small amount of inflammatory cells invasion, the alveolar interval slightly thickened; At the ninth hour, the lung tissue structure was clear but the alveolar structure was incomplete, the alveolar walls were broken and damaged, part of the alveolar lumen was narrowed with more inflammatory cells invaded,also the alveolar interval thickened; At