结节性甲状腺疾病dapk,rarβ2基因甲基化和mrna表达及临床意义-methylation and mrna expression of dapk and rar β 2 gene in nodular thyroid disease and its clinical significance.docx

下载文档 降价啦

2
0
约3.5万字
约 42页
2018-05-29 发布于上海
举报
版权申诉
保障服务

结节性甲状腺疾病dapk,rarβ2基因甲基化和mrna表达及临床意义-methylation and mrna expression of dapk and rar β 2 gene in nodular thyroid disease and its clinical significance.docx

1、本文档共42页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

结节性甲状腺疾病dapk,rarβ2基因甲基化和mrna表达及临床意义-methylation and mrna expression of dapk and rar β 2 gene in nodular thyroid disease and its clinical significance

group(P=0.000,0.003,P0.05),significantdifference;RARβ2geneinthethyroidcancer,thyroidinflammationinmergerhashimotonodulesmRNAexpressreduceorexpressismissing,andnormalcontrolgroup(P=0.000,comparedto0.002,P0.05)havesignificantdifferences.(5)DAPK,RARβ2geneexpressiontoreduceorlackandgenepromoterareaabnormalmethylationinvolved;GenemethylationandmRNAexpressionwasnegativelycorrelated.Conclusion(1)DAPK,RARβ2geneinthethyroidcancerinperipheralbloodarehighinmethylationratewhilethemRNAexpressisreducedorlostinexpression;thelackDAPKgeneinthethyroidglandtumorshashighrateofmethylation,methylationrateslowerthanthyroidcancergroup,reducemRNAexpression;RARβ2geneinthethyroidinflammationintheperipheralbloodishashimotohighmethylationstate,reducemRNAexpression.(2)Inthethyroidcancer,genemethylationofDAPK,RARβ2hasnosignificantdifferenceswithgender,age,sizeofthenodule,butisrelevanttonodulescalcificationandnecklymphnodeenlargement.(3)JointtestRARβ2,DAPKgenemethylationstatecanobviouslyincreasesensitivityofthyroidcancer,whichmaybecomeathyroidcancerearlydiagnosisofeffectiveindex.(4)ThelossofgeneexpressioninDAPK,RARbeta2isrelevanttogenepromoterareaabnormalmethylation.Keywords:DAPK;RARβ2;Nodularthyroiddisease;Methylation;mRNAexpression;PeripheralbloodDirectedby:Prof.WEIFengApplicantforMasterdegree:WANGZhaoxia(DepartmentofEndocrinology)(TheFirstAffiliatedHospital,BaotouMedicalCollege,014060.China)..1.2...2...2...3..5..10..11..12..12..12..12.12.12.12..14..18..23.24.2736.37.38..38目录1前言.............................................................11.1DAPK，RARβ2基因甲基化及表达情况的提出11.2DAPK，RARβ2基因甲基化及表达的目的与意义22材料与方法.....................................................22.1试验材料.....................................................22.2研究对象.....................................................32.3甲基化特异性PCR52.4基因组RNA抽提及逆转录多聚酶链反应（RT-PCR）103统计学分析.......................................................113.1分析不同甲状腺疾病分组与对照组甲基化率及mRNA表达123.2不同甲状腺疾病分组甲基化与临床指标的关系.....................123.3分析甲状腺癌分组内基因表达和甲基化关系......................