金线莲离体快繁及人工栽培条件分析-analysis of in vitro rapid propagation and artificial cultivation conditions of anoectochilus roxburghii.docxVIP

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金线莲离体快繁及人工栽培条件分析-analysis of in vitro rapid propagation and artificial cultivation conditions of anoectochilus roxburghii.docx

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金线莲离体快繁及人工栽培条件分析-analysis of in vitro rapid propagation and artificial cultivation conditions of anoectochilus roxburghii

活力差异达极显著水平。金线莲是一种重要的药用植物，其药用成分总黄酮的含量差异达极显著水平，A2B1 组合中金线莲药用成分总黄酮的含量最高。对金线莲各项生理指标与总黄酮的含量做相关性及通径分析，发现，金线莲总黄酮含量与丙二醛含量、可溶性糖含量相关性不显著。硝酸还原酶活力与总黄酮的含量成显著正相关，叶绿素总含量、根系活力及可溶性蛋白含量与总黄酮的含量成极显著正相关。关键词：金线莲；离体快繁；人工栽培；条件；生理生化指标Study on Micropropagation and Artificial Cultivation ofAnoectochilusroxburghii(Wall.) Lindl.AbstractThe factors to establish the aseptic regeneration system of Anoectochilus roxburghii, such as mercuric chloride concentration, sterilization time, hormone levels, light intensity and temperature, etc. were studied. Orthogonal design was implemented in studying seedling culture, induced protocorm medium and the effect of different hormone in rooting culture medium. At the same time, preparation technique of Anoectochilusroxburghii synthetic seeds was studied. The resultsshowed that: the best disinfection measures was the HgCl2 level 0.15%、4 min; the significant role inseedling was the MS + 6-BA 0.5 mg/L + NAA 0.5 mg/L culture medium and 3000-3500 lx illumination intensity, 23℃, 12h /d; the significant role in protocorm induced was the MS + 6-BA 1.0 mg/L + NAA 0.05 mg/L culture medium and 1000-1500 lx illumination intensity, 23℃, 12 h/d;Studied the rooting culture medium, The results showed that the significant role was the 1/2 MS + IBA1.0 mg/L + NAA 0.1 mg/L + AC 0.25%. The orthogonal design study on preparation technique of Anoectochilusroxburghii synthetic seeds showed that the seed germination rate and seedling rate have strong correlation with treatment method. The seed germination rate and seedling rate were maximized in A4B1C2D2E1 case.Effects of transplanting tissue culture substrate and light intensity were also studied. Results showed that the maximum survival rate was 94% in Humus: Land: Perlite= 2:2:1 matrix and placed in the interior(Transmittance 6%) after 30 days.Two-factor experiment design was carried out to study the effects of culture media and fertilizer for survival plantlets after transplanting. It was found that culture media an