雷公藤甲素对急性肺损伤大鼠肺内abca1表达影响-effect of triptolide on expression of abca 1 in lung of rats with acute lung injury.docxVIP

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雷公藤甲素对急性肺损伤大鼠肺内abca1表达影响-effect of triptolide on expression of abca 1 in lung of rats with acute lung injury.docx

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雷公藤甲素对急性肺损伤大鼠肺内abca1表达影响-effect of triptolide on expression of abca 1 in lung of rats with acute lung injury

StudyfortriptolideregulatedtheexpressionofATP-bindingcassettetransporterA1inacutelunginjuryofratsAbstractPartⅠ.EstablishmentofanimalmodelofLPS-inducedacutelunginjury(ALI)onratsPurposeThisstudywassupposedtoassessthereliabilityofLPS-inducedacutelunginjury(ALI)onrat.MethodsThirtymaleSDrats,withbodyweightranging200~250g,wererandomizedintotwogroups,withfifteenineach.Theratswereintravenouslyadministered5mg/kgLPS(Modelgroup)ortheequivalentamountofnormalsaline(Controlgroup)throughtailvein.Arterialbloodwasrepeatedlysampledforblood-gasanalysisat1hrbeforeand3,6,12hrafterinjection.Immediatelyafterlastbloodsampling,ratswereexecutedbydecapitation.Thelowerlobeofrightlungwasharvestedforparaffinslice,HEstaining,andhistopathologicalexaminationunderlightmicroscope.Andthemiddlelobeofrightlungwassavedforcalculatingratioofwettodry(W/D).ResultsAsforblood-gasanalysis,PaO2inModelgroupshowedstatisticallysignificantlowerthanthatinControlgroupat3,6and12hr(P0.05).AndPaO2at12hrinModelgroupdecreasedby46.01%ofbaseline.Histopathologicalexaminationoflung,Gross:thelunginControlgroupdisplayednormalsize,pink,glazedsurface,finesection,withnoexudationorcongestion.WhilethelunginModelgroupappearedhypertrophy,wine-colored,subcapsularllyscatteredbleedingspots,loosetexture,withyelloworreddishextravasate.LM:thelunginControlgroupdisplayedintactstructure,clearalveoliwithfullcavity,smoothwall,andevenspace,noexudationorleucocyteinalveoli,andonlyinfrequentleucocyteatalveolusintervals.WhilethelunginModelgroupappearedmassiveinfiltrationofinflammatorycells,conpicusousdilationandcongestionofcapillary,incompletealveoliwithdamagedwallandmassiveerythrocyte,neutrophil,macrophage,extravasate,andevenmicrothrombosisinbetween,augmentofalveolusdistance,andsomealveoluscollapse.Inall,thelowerlobeofrightlunginModelgroupexhibitedstatisticallysignificantinjuryataspectsofcapillarycongestion,neutrophilinfiltration,andincreasedalveolusdistance,ascomparedtoControlgroup(P0.05).TheW/DratioofmiddlelobeofrightlunginModelgroupdenotedstatisti