冷胁迫下茶树蛋白组学和转酮醇的分析-analysis of proteomics and transketol of tea tree under cold stress.docxVIP

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冷胁迫下茶树蛋白组学和转酮醇的分析-analysis of proteomics and transketol of tea tree under cold stress.docx

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冷胁迫下茶树蛋白组学和转酮醇的分析-analysis of proteomics and transketol of tea tree under cold stress

AbstractTea plant (Camellia sinensis) is a kind of important cash crops in China, as low temperature is a significant abiotic stress constraint restricing crop growth, production and quality as well as geographical distribution. It could cause unrecoverable damage and even death to tea plant under serious condition. Two cultivars of tea plant (Longjing 43 and Wancha 91) which growed wildly in Anhui province were used as materials treated with different low temperature to study their genotypic responses and physiological mechanisms response to cold stess.To apply technology of tea plant physiology and biochemistry combined with the low temperature stress on biological and biochemical responses on new shoots ofLongjing 43 and Wancha 91 treated at 4℃ and 0℃ while 25℃ is the control group,then culculate the difference of cell membrane permeability, soluble protein content, soluble carbohydrate content, MDA content, proline content and activities of protective enzymes. The results showed that cell membrane permeability, soluble protein content, soluble carbohydrate content, MDA content, were increased, and activities of protective enzymes had enhanced, while few changes of proline content.By using 2 DE-GEL method to analyze the cold-induced proteins in Longjing 43 seedlings under 4℃, and found 46 differentially expressed protein spots, then 7 spotswere chosen to identify with MALDI-TOF-TOF-MS successfully. Six of these proteins were fragments of ribulose-1, 5-bisphosphate carboxylase/oxygenase has effect on Calvincycle in photosynthesis, one was ribulose activase, the test is transketolase (TK) which could play important role in the pentose phosphate pathway, metabolisms of carbon, and resisitance.Isolated the full-length cDNAs of TK from Longjing 43 using the technique of RACE, designating with TK in others plant, and found the deduced protein TK contains a highly conserved basic active domain and C-terminal region of TPP super-family.Procaryotic expression vector