利用废弃碳源异养培养小球藻制备生物柴油的分析-analysis on preparation of biodiesel by heterotrophic cultivation of chlorella with waste carbon source.docxVIP

AbstractRecent years, environmental problems were emerging due to the extended applications of fossil fuel and the greenhouse gases emissions. The development of renewable energy has become a research interest in energy and the environmental field. Biodiesel is a renewable energy source with the advantages of renewable, non-toxic, biodegradable. However, two main problems in the applications of biodiesel were the high cost and the low yield. In order to reduce the cost of microalgal culture and improve the biodiesel yield, wasted carbon sources were investigated as the alternative carbon source to glucose in this research. Meanwhile, organic wastewater and waste sludge produced during wastewater treatment contain carbon sources, nitrogen and phosphorus nutrition elements and trace elements, as well as harmful ingredients and biodegradable pollutants.Two types of waste carbon sources were chosen in this research, ie.effluent from organic wastewater acidification and sludge hydrolyzate. The effluent was taken from an anaerobic CSTR (Status of Continuous Stirred Tank Reactor) treating synthetic molasses wastewater. Sludge taken from a sanitary wastewater treatment plant in Harbin was to give the hydrolyzate.The COD concentration of the effluent from the stably operated CSTR was about 5200mg/L, which contained 40% of volatile fatty acids (VFAs). The main components of the effluent were ethanol, acetic acid, propionic acid, butyric acid, valeric acid, and sugar unused, and some macromolecules. No phosphorus and nitrogen was detected in the effluent. The composition of the sludge hydrolyzate was mainly VFAs and the average COD concentration of the sludge hydrolyzate was 3838mg/L. VFAs accounted for 60% of the total COD concentration. Nitrogen concentration of the sludge hydrolyzate was 264.82mg/L and total phosphorus concentration was 205.31mg/L.Experiments were conducted to compare whether the mediums of the CSTR effluent and sludge hydrolyzate culture were feasible to h