凉粉草质量控制技术分析-analysis of quality control technology of mesona chinensis.docxVIP

凉粉草质量控制技术研究中文摘要目的：通过对凉粉草质量控制技术的研究，为凉粉草质量标准的确定提供依据。方法：通过原植物鉴定、性状鉴定、显微鉴定、薄层鉴别的方法进行定性研究；按2010年版《中国药典》进行药材水分、灰分、浸出物等限量研究；采用HPLC法，以咖啡酸为指标性成分，对其进行含量测定并初步进行了指纹图谱研究；采用ICP-MS法同时测定7种微量元素的含量。结果：1.原植物形态、性状特征、显微特征与薄层色谱均具有鉴别意义。2.药材水分含量应控制在0.073%以内；总灰分含量不得超过16.27%；酸不溶性灰分含量不得超过1.86%；水溶性浸出物含量不得低于7.60%；3.凉粉草中咖啡酸含量测得值在0.2399mg·g-1～0.6365mg·g-1之间；4.初步建立了凉粉草药材HPLC指纹图谱，筛选出18个共有峰；5.建立了凉粉草中微量元素的含量测定方法，为其安全评价提供参考依据。结论：在检查、鉴别、含测等方面对凉粉草进行初步研究，为凉粉草的质量标准拟定提供参考依据。关键词：凉粉草；含量测定；指纹图谱；质量控制StudyonQualityControlTechnologyofMesonachinensisBenthABSTRACTObjective:QualitycontroltechnologywasresearchedtoprovidereferencesforqualitystandardsofMesonachinensisBenth.Methods:Originidentification，macroscopicidentification，characteridentificationandthin-layerchromatographyidentificationwereadoptedtoresearchthequalitativeidentificationofMesonachinensisBenth．AccordingtoChinesePharma-copeia(2010)，thecontentlimitationcontrolofwater，ashandwaterextractsweredetermined．ContentdeterminationofcaffeicacidwhichwasthetargetcomponentandpreliminarystudyonthefingerprintsofMesonachinensisBenthwereestablishedbyHPLC;thecontentof7elementswassimultaneouslydeterminedbyICP-MS.Results:1.Thepharmacognosicalcharacteristicsoforiginidentification,characteridentification,macroscopicidentificationandthin-layerchromatographyidentificationaremeaningfultoidentifyingMesonachinensisBenth.2.ThewaterinMesonachinensisBenthcouldn’tbehigherthan0.073%;thetotalashcouldn’tbehigherthan16.27%；theacid-insolubleashcouldn’tbehigherthan1.86%;thewaterextractscouldn’tbelowerthan7.60%.3.ThecontentofcaffeicacidinMesonachinensisBenthwerearound0.2399mg·g-1～0.6365mg·g-1．4.HPLCfingerprintswerepreliminarilyestablishedonMesonachinensisBenthand18commonpeakswereselected．5.ThecontentdeterminationoftraceelementswasestablishedtoprovidethereferencesofsecurityevaluationforMesonachinensisBenth.Conclusion:MesonachinensisBenthwaspreliminarilystudiedondiscrimination,identificationandmeasurementetal.toprovidethereferencesforqualitystandardsofMesonachinensisBenth.Keywords:Mesonachinen