牙髓成牙本质细胞层毛细淋巴管透射电镜研究.docVIP

牙髓成牙本质细胞层毛细淋巴管透射电镜研究 　　【摘要】 目的 研究牙髓成牙本质细胞层中有无毛细淋巴管及其超微结构特点。方法 取牙根发育完成的健康恒前磨牙15颗,纵向劈开后在2.5%戊二醛中4℃固定1 h,完整地取出牙髓,切成截面积约为1 mm?2,长约3 mm的小长条,再在同一固定液中固定2 h,后固定,包埋,半薄切片定位,选取5例牙髓进一步作超薄切片,铀铅染色,用透射电镜观察。结果 在成牙本质细胞层中观察到具有典型毛细淋巴管超微结构特点的结构。结论 在牙根发育完成的年轻恒前磨牙成牙本质细胞层中有毛细淋巴管,其具有与其他组织毛细淋巴管基本相似的超微结构特征。 　　【关键词】 前磨牙;成牙本质细胞;毛细淋巴管;透射电镜 　　Studies on the Lymphatic Capiliaries in the Odontoblast Layer ofPulp with Transmission Electron Microscope. 　　GONG Yan-hong,WANG Zhu-ping,WANG Duo,et al.Department of Stomatology,Qianfoshan Hospital of Shandong Province,Jinan 250014,China 　　【Abstract】 Objective To study the lymphatic capiliaries in the odontoblast layer of pulp.Methods 15 healthy premolars were employed in the experiments whose roots had developed.They were cleaved longitudinally as soon as they were extracted and put into 2.5%glutaraldehyde soulution for 1 hour at 4℃.The pulps were taken out and cut into little pieces and re-immersed in the same soulution for 2 hours.Then they were postfixed,dehydrated and embedded.And semithin sections were made.5 pulps were chosen to make ultrathin sections and observedby transmission electronmicroscope(TEM).Results In the odontoblast layer,structures which had typical characters of lymphatic capiliaries were found with TEM.Conclusion There are lymphtic capiliaries in the odontoblast layer of pulps which are young and healthy.The characters of lymphtic capiliariy are similar to those of other tissues. 　　【Key words】 Premolar; Odontablast; Lymphatic capiliary; Transmission electron microscope 　　 　　牙髓中存在淋巴管已是共识,许多学者用多种观察法对牙髓淋巴管的分布做过研究[1-5],但在成牙本质细胞层中是否有毛细淋巴管目前尚存在争议。为了深入探讨这一问题,本文应用透射电镜对此进行了研究。 　　1 材料和方法 　　1.1 材料 　　1.1.1 标本收集 选取因正畸而需要拔除恒前磨牙的患者9例,年龄14~22岁,男5例,女4例,取得牙根发???完成的健康恒前磨牙15颗。 　　1.1.2 试剂与仪器 常规透射电镜观察所用试剂及透射电镜(JEM1200EX型,日本)。 　　1.2 方法 15颗恒前磨牙拔出后立即纵向劈开,放在2.5%戊二醛中4℃固定1 h,完整地取出牙髓,将其切成截面积约为1 mm?2,长约3 mm的小长条,再在同一固定液中4℃固定2 h。1%锇酸后固定1 h,脱水,包埋,半薄切片,甲苯胺蓝染色定位,选用5颗成牙本质层结构良好且在该层细胞中有难以确定是毛细血管还是毛细淋巴管的管状结构的牙髓,进一步作超薄切片,醋酸铀、枸橼酸铅染色,JEM1200EX透射电镜观察。 　　2 结果