柳树对砷的吸收和积累特性及其对菲的耐受性的分析-absorption and accumulation characteristics of arsenic in willow and its tolerance to phenanthrene.docxVIP

AbstractPhytoremediationisagrowingfieldofresearchinenvironmentalstudiesbecauseoftheadvantagesitoffers,suchasenvironmentalfriendliness,costeffectiveness,andsustainability.Willow(Salixsp.)whichhaslargebiomassislow-costandfast-growing.Itisanimportantmultipurposetreespeciesthatshowshighresistancetosalt,alkali,drought,andwater-loggingstress.What’smore,willowhasaremarkablecapacitytoconcentratetoxicheavymetals.Thecurrentstudyaimstoexplorethepotentialofwillowtoremediatearsenic-contaminatedwaterandsoilinhydroponicsculturesandpotcultures.Inthecurrentstudy,fivewillowspecieswereevaluatedandcomparedtoinvestigatetheaccumulationabilityofarsenicfromwaterandsoil.Allthewillowsinourstudywerecultivatedingreenhouseorartificialclimateincubator.Fivewillowspecies,namely,SalixjiangsuensisCLJ-172,Salixmatsudana,SalixintegraYizhibi,SalixinregraWeishanhu,andSalixmongolicawerecultivatedinhydroponicscultures.Thearsenictolerance,uptakeinflux,arsenicaccumulation,andphytofilterabilityofwillowsweretestedinthecurrentresearch.SalixintegraYizhibihadthehighestrootarsenicconcentrationat604.66mg·kg-1,whereasSalixmatsudanahadthelowestat321.83mg·kg-1amongthefivetestedspeciesexposedto80μMarsenateAs(V)).As(V)influxintoS.integraYizhibiwasadequatelydescribedbytheMichaelis–Mentenequation,whereVmax(±SE)valuewas3.42±0.94nmol·kg-1DWmin-1andKm(±SE)valuewas14.82±0.89nM.ThearsenicandphosphorusconcentrationsintherootsofS.integraYizhibiincreasedwithincreasingsolutionAs(V)concentrationupto320μM.Within180h,S.integraYizhibidecreasedtheAs(V)concentrationinthesolutionfrom290μg·L-1to142μg·L-1,witharemovalrateof50%.Fourwillowspecies,namely,SalixjiangsuensisCLJ-172,Salixmatsudana,SalixintegraYizhibi,andSalixmongolicawerecultivatedinpotcultures.Allthewillowspeciesdidnotshowtoxicsymptomsofetiolationandscorchingfromitsappearance,growthofthewillowspeciesandnutrientintheplantsshowednosignificantdifferencesafterexposuretoAs(V)concentrationofrangedfrom0to50mg·kg-1for160d.TheAsuptakeintheroots(P0.01)andstemsofwillowspeciesincreasedwith