一种简易兔心肌梗死模型建立.docVIP

一种简易兔心肌梗死模型建立 　　【摘要】目的：探讨一种建立兔急性心肌梗死(MI)模型的简易方法。方法：健康成年雌兔20只，在无气管插管和呼吸机的情况下，正中纵行剪开下段胸骨，同时保持肋骨和胸膜腔完整，在左心耳缘下5~10 mm内缝扎左室支(LVB)，建立MI模型，结扎LVB 30 min后查心电图(ECG)。4周后，行ECG、心脏彩超检查。符合MI心脏彩超和ECG造模成功标准的兔，视为造模成功；并制作心肌病理切片，行HE染色、Masson三色染色，观察病理变化。结果：结扎LVB后，兔ECG出现心肌缺血改变；术后4周动物存活率85%，造模成功率70%，左室舒张末内径(LVEDD)、左室收缩末内径(LVESD)、左室缩短分数(LVFS)与术前差异均有统计学意义(P 　　【关键词】心肌梗死；兔动物模型；左室支 　　 　　A Simple Rabbit Model of Myocardial Infarction/YANG Yong-hui，LI Tong，ZHAO Yue，et al.//Medical Innovation of China，2012，9(10)：001-003 　　【Abstract】Objective：To prepare a simple，reliable rabbit myocardial infarction model.Methods：Carefully incising the lower sternums of 20 healthy adult female rabbits along the midline，without endotracheal intubation and ventilator，maintaining the integrity of the ribs and pleural cavity，and ligated left ventricular branch at 5~10 mm below the left atrial appendage，to establish the model of myocardial infarction.4 weeks later，the survival rabbits were detected by the ECG and echocardiography.Rabbits which complied with the model standard of the ECG and echocardiography were considered as successful MI models.Made some myocardial pathological biopsy，stained with HE and Masson trichrome，then，observing pathological changes.Results：14 rabbits had myocardial ischemia changes in ECGand decreased cardiac function in echocardiography 4 weeks after ligation of LVB.The survival rate and success rate of model rabbits were 85% and 70% after postoperative 4 weeks respectively.The statistically significant differences were found in the left ventricular end-diastolic diameter，left ventricular end-systolic diameter，and left ventricular fractional shortening，compared with preoperative(P 　　1.3模型监测及评价 　　1.3.1ECG监测实验兔四肢及胸前连接电极，分别于冠状动脉结扎前、结扎后30 min、建模后4周行ECG检查，记录Ⅱ导联。冠状动脉结扎后30 min较结扎前出现典型ST段弓背抬高，T波高耸；同时建模后4周出现病理性Q波作为建模成功的标准之一。 　　1.3.2心脏彩超检查实验兔于造模前及造模后4周行心脏彩超检查，测定心率(heart rate，HR)，左室舒张末内径(left ventricular end-diastolic diameter，LVEDD)，左室收缩末内径(left ventricular end-systolic diameter，L