低频电针足三里对酒依赖大鼠饮酒行为影响.docVIP

低频电针足三里对酒依赖大鼠饮酒行为影响 　　【摘 要】 目的：研究低频电针（2Hz）足三里对酒依赖大鼠饮酒行为的影响。方法：共24只SD大鼠（酒依赖模型组18只，对照组6只）。采用6%(v/w)的酒精水溶液作为SD大鼠唯一的饮水来源共30天建立酒精躯体依赖模型，进一步将其分为戒断组、捆绑组、电针组各6只大鼠。分别对酒精依赖大鼠进行急性（电针一次）和慢性（电针六次）低频电针足三里处理，观察急慢性电针对大鼠饮酒量和酒精偏爱程度 （酒精/水自由选择）的影响。结果：急性电针足三里处理大鼠饮酒量和酒精偏爱组间比较差异有显著性(F (2,15)=7.034, P =0.007；F (2,15)=4.810, P =0.024)，电针组饮酒量和酒精偏爱较对照组（捆绑对照和非捆绑对照）均降低，其中三组饮酒量和酒精偏爱均值和标准差分别为（4.2±0.4，9.6±0.9，8.0±0.7）及（4.3±0.2，7.5±0.7，7.8±0.7）；差异有统计学意义(P＜0.001)。慢性电针足三里处理后大鼠饮酒量和酒精偏爱程度与对照组（捆绑对照和非捆绑对照）比较均无显著性差异。结论：急性低频电针足三里能抑制酒精依赖大鼠的饮酒行为。 　　【关键词】 成瘾行为；酒依赖； 大鼠；电针； 足三里穴；饮酒行为；动物试验 　　中图分类号：Q42、R595.6 文献标识码：A 文章编号：1000-6729(2008)003-0157-07 　　Effects of Low Frequency Electroacupuncture at Zu SanLi on Ethanol Taking Behavior 　　in Ethanol Dependent Rats 　　WANG YuHong, YU JiangLiang, HUANG JinSong, et al 　　Mental Health Institute, The 2nd Xiangya Hospital of Central South University, Changsha 410011 　　【Abstract】 Objective: To evaluate the effects of low frequency electroacupuncture (EA) at Zu SanLi on ethanol taking behavior in ethanol dependent rats. Methods:18 Rats were made ethanoldependent by supplying ethanol solution (6%, v/w) as the sole drinking source for 30 days. Total ethanol intake and ethanol (6%)/water choice preference were measured after acute （one time）or chronic (six times) EA at Zu SanLi. Two control groups were set as constrained control, nonconstrained control.Results: There were significant differences in both total amount of ethanol intake and ethanol preference (ethanol/water free choice) among three groups after acute EA at Zu SanLi (F (2,15)=7.034, P =0.007; F (2,15)=4.810, P=0.024). The rats received acute EZ had lower ethanol intake and ethanol preference than the two control groups ( 4.2±0.4, 9.6±0.9, 8.0±0.7 and 4.3±0.2, 7.5±0.7, 7.8±0.7;P＜0.001), but this effects were not observed after chronic EA treatment.Conclusion:Acute low frequency EA at Zu SanLi could attenuate ethanol intake and preference in ethanoldependent rats. 　　【Key words】 addiction; ethanol dependent ra