灰绿黄堇总生物碱对实验性溃疡性结肠炎作用研究.docVIP

灰绿黄堇总生物碱对实验性溃疡性结肠炎作用研究 　　【摘要】 目的 研究灰绿黄堇总生物碱(ACAM)对小鼠结肠炎的作用及其机制。方法 实验设正常、模型、柳氮磺胺吡啶组(SASP，520 mg/kg)和ACAM组(100、200、400 mg/kg)。正常组小鼠饮用蒸馏水，其余组自由饮用4%葡聚糖硫酸钠(DSS)水溶液，同时分别灌胃给予溶剂或干预药物(0．2 ml/10 g,1次/d×7 d)。记录小鼠疾病活动指数(DAI)；测定结肠组织MDA含量，SOD、MPO活性及ICAM-1、NF-κB p65表达水平。结果 模型组DAI显著增高，结肠黏膜损伤严重；MDA舍量、MPO活性及ICAM-1和NF-κB p65表达明显升高。SOD活性下降(P 　　【关键词】葡聚糖硫酸钠；灰绿黄堇总生物碱；结肠炎 　　?? 　　 　　The effect of alkaloids of corydalis adunca maxim on experiment ulcerative colitis induced by dextran sulfate sodium 　　 　　MA Guo-zhong.Department of General Surgery，The Third Hospital of Mianyang，Sichuan Province 621000，China 　　? 　　【Abstract】 Objective To investigate the effect of alkaloids of corydalis adunca maxim(ACAM)on experimental ulcerative colitis in mice.Methods BALB/C mice of 6 groups were allowed to drink either 4%dextran sulfate sodium(DSS)solution or distilled water freely with different doses of ACAM(50,100,200 mg/kg)or salicylazosulfapyridine(SASP,520 mg/kg)，and solvent(0．2 ml/10 g)once a day for 7 days,respectively.The symptom of ulcerative colitis was evaluated by disease activity index(DAI),Myeloperoxidase(MPO)and superoxide dismutase(SOD)activities and malondialdehyde(MDA)content were determined by HE staining and immunohistochemistry of expressions of NF-κB p65 and intercellular adhesion molecule 1(ICAM-1)proteins to observe the damage of colon tissues and.possible mechanisms.Results DAI,MPO activity,MDA content and expressions of ICAM-1 and NF-κB were markedly increased,while SOD activity decreased in DSS-treated mice.Treatment of different doses of ACAM or SASP in mice decreased significantly DAI,MPO activity and MDA content,improved histological changes of colon tissues,blunted the expressions of NF-κB p65 and ICAM-1 proteins,and promoted SOD activity.Conclusion ACAM has excellent therapeutic effect on ulcerative colitis caused by DSS.The possible mechanism may be related to its antioxidant and anti-inflammatory activities associated with inhibiting the NF-κB activation and ICAM-1