祛异康对子宫内膜异位症模型大鼠血管内皮生长因子影响.docVIP

祛异康对子宫内膜异位症模型大鼠血管内皮生长因子影响 　　摘 要：目的：观察中药祛异康对子宫内膜异位症大鼠在位、异位子宫内膜组织血管内皮因子（VEGF）的表达情况，探讨其治疗子宫内膜异位症的机理。方法：将40只健康雌性SD大鼠分为4组: A组只行开关腹,B、C、D组均行内膜移植，造模完成后,A、B组以生理盐水灌胃，C、D组分别给予西药丹那唑、中药祛异康灌胃， 4周后于动情期处死，用免疫组化方法检测在位、异位子宫内膜组织VEGF的表达情况。结果:在降低异位内膜组织VEGF的表达上丹那唑组、祛异康组与模型组比较均有显著性差异(P0.05)。结论:祛异康能明显抑制子宫内膜异位症模型大鼠异位内膜的生长,使异位内膜萎缩,并能明显抑制异位内膜周围血管的形成；其治疗机理可能是通过降低VEGF在异位内膜组织中的表达而实现的。? 　　关键词：子宫内膜异位症；大鼠模型；血管内皮因子；中药祛异康；实验研究 　　中图分类号：R285.5文献标识码：A 　　文章编号：1673-7717(2008)05-0979-03? 　　Effect of Quyikang on VEGF of Endometriosis in Rats 　　? 　　YANG Jian?bing?1，MENG Qiao?rong?2，CHEN Mei?1，LIU Dong?ping?1，ZHU Li?hong?1? 　　(1.Shaanxi University of Chinese Medicine，Xianyang 712046，Shaanxi，China；2.Tibet National College，Xianyang 712000，Shaanxi，China) 　　Abstract:Objective:to observe effect of Quyikang on VEGF expression in Emdometrium and endometriotic tissue in rats and explore the probable mechanisms.Methods:40 female SD rats divided into four groups,A group is control group,B,C and D groups are established of endometriosis modle rat.A,B groups were fed with 0.9%Nacl,C group was fed with Danazol fliud,D group fed with Quyikang fliud,After four weeks,all rats were skilled at the metaestrs stages.VEGF expression in Emdometrium and endometriotic tissue of each group were analyzed by immunohistochemistry mdthod.Results:compared with modle groups,the endometriotic tissues volume of Quyikang group and Danazol group were also obviously reduced(P0.05).Conclusion:Quyikang could inhibit the growth and proliferation of endometriotic tissue in rats modle,and eliminate the neovascular networks on the surface of transplants:VEGF expression in endometriotic tissue was obviously reduced by Quyikang treatment. 　　Keywords:endometrisis;rat modle;vscular endothelial growth factor(VEGF);Quyikang;experiment study 　　 　　子宫内膜异位症（endometriosis EMS）是一种具有种植生长、侵袭、远处转移和易复发等类似恶性肿瘤特点的疾病，故临床常表现为盆腔和腹部疼痛、痛经、性交痛、月经失调、不孕等，是育龄女性的常见多发病，严重影响妇女健康和生活质量[1]。本研究通过动物实验，从异位症模型大鼠在位、异位子宫内膜组织血管内皮因子（Vascular endothelial growth factor，VEGF）的表达情